Cessation of cytokinesis in Schizosaccharomyces pombe during growth after release from high hydrostatic pressure treatment

• Published in: Bioscience, biotechnology, and biochemistry Vol. 72; no. 1; pp. 88 - 93
• Main Authors: Arai, S.(Nihon Univ., Fujisawa, Kanagawa (Japan). Coll. of Bioresource Sciences), Kawarai, T, Arai, R, Yoshida, M, Furukawa, S, Ogihara, H, Yamasaki, M
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.01.2008; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: actin; Actins - metabolism; ALIMENTOS; Biological and medical sciences; biotechnology; Cell Count; CELL DIVISION; Cell Survival; cytokinesis; Cytokinesis - physiology; cytoskeleton; Cytoskeleton - physiology; Cytoskeleton - ultrastructure; DIVISION CELLULAIRE; DIVISION CELULAR; Escherichia coli; ESTERILIZACION; FOODS; Freezing; Fundamental and applied biological sciences. Psychology; growth retardation; HIGH PRESSURE TECHNOLOGY; high pressure treatment; Hydrostatic Pressure; Kinetics; MICROSCOPIA; MICROSCOPIE; MICROSCOPY; microtubules; myosin; Myosins - metabolism; PRODUIT ALIMENTAIRE; Schizosaccharomyces - cytology; Schizosaccharomyces - growth & development; SCHIZOSACCHAROMYCES POMBE; Schizosaccharomyces pombe Proteins - metabolism; STERILISATION (GERMES); STERILIZING; TECHNOLOGIE HAUTE PRESSION; TECNOLOGIA ALTA PRESION; Ascomycota; Fungi; Yeast; Treatment; Growth; Hydrostatic pressure; high pressure treatment; Schizosaccharomyces pombe; Cytoskeleton; Release; High pressure
• ISSN: 0916-8451; 1347-6947; 1347-6947
• DOI: 10.1271/bbb.70451

On the basis of our previous study concerning the effect of high hydrostatic pressure treatment (HPT) on Escherichia coli FtsZ ring (bacterial cytoskeleton) formation, we aimed to determine the effect of HPT on the growth properties of a representative eukaryotic microbe, Schizosaccharomyces pombe, in relation to the behavior of genuine cytoskeletons. Microtubules were visualized with GFP-linked alpha-tubulin. Actin-related cytoskeletons were fluorescently stained with rhodamine-phalloidin. We observed growth retardation of about 10h in post growth after HPT (75 MPa, 30 min, 28 deg C), which caused only a little loss of viable cells. In accordance with the period of growth retardation, cessation of cytokinesis and disappearance of the contractile ring (composed of actin, myosin II, and other proteins), directly participates in cytokinesis, continued for 18 h after HPT. On the other hand, the microtubules disappeared only for 6 h after HPT. Based on these observations, the contractile ring was the site most sensitive to HPT resulting in the cessation of cytokinesis.