Recombinant Pvs48/45 Antigen Expressed in E. coli Generates Antibodies that Block Malaria Transmission in Anopheles albimanus Mosquitoes

• Published in: PloS one Vol. 10; no. 3; p. e0119335
• Main Authors: Arévalo-Herrera, Myriam, Vallejo, Andrés F., Rubiano, Kelly, Solarte, Yezid, Marin, Catherin, Castellanos, Angélica, Céspedes, Nora, Herrera, Sócrates
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16.03.2015; Public Library of Science (PLoS)
• Subjects: Animal species; Animals; Anopheles; Anopheles - immunology; Anopheles - parasitology; Antibodies, Protozoan - metabolism; Antigenicity; Antigens; Antigens, Protozoan - genetics; Antigens, Protozoan - immunology; Aotidae - immunology; Aotidae - parasitology; Aquatic insects; Blocking; Blood & organ donations; Disease transmission; E coli; Epitopes; Epitopes - immunology; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Ethics; Fertilization; Genomics; Haplorhini; Health aspects; Humans; IgG antibody; Immunization; Immunofluorescence; Immunogenicity; Immunoglobulin G; Immunoglobulin G - metabolism; Immunoglobulins; Indigenous species; Infection; Laboratory animals; Malaria; Malaria Vaccines - administration & dosage; Malaria Vaccines - genetics; Malaria Vaccines - immunology; Malaria, Vivax - prevention & control; Malaria, Vivax - transmission; Malaria, Vivax - veterinary; Male; Mice; Mice, Inbred BALB C; Monkeys; Monkeys & apes; Mosquitoes; Parasites; Plasmodium; Plasmodium vivax; Plasmodium vivax - genetics; Plasmodium vivax - immunology; Proteins; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Studies; Vaccine development; Vaccines; Vector-borne diseases; Colombia
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0119335

Transmission of malaria parasites from humans to Anopheles mosquitoes can be inhibited by specific antibodies elicited during malaria infection, which target surface Plasmodium gametocyte/gamete proteins. Some of these proteins may have potential for vaccine development. Pvs48/45 is a P. vivax gametocyte surface antigen orthologous to Pfs48/45, which may play a role during parasite fertilization and thus has potential for transmission blocking (TB) activity. Here we describe the expression of a recombinant Pvs48/45 protein expressed in Escherichia coli as a ∼60kDa construct which we tested for antigenicity using human sera and for its immunogenicity and transmission blocking activity of specific anti-mouse and anti-monkey Pvs48/45 antibodies. The protein reacted with sera of individuals from malaria-endemic areas and in addition induced specific IgG antibody responses in BALB/c mice and Aotus l. griseimembra monkeys. Sera from both immunized animal species recognized native P. vivax protein in Western blot (WB) and immunofluorescence assays. Moreover, sera from immunized mice and monkeys produced significant inhibition of parasite transmission to An. Albimanus mosquitoes as shown by membrane feeding assays. Results indicate the presence of reactive epitopes in the Pvs48/45 recombinant product that induce antibodies with TB activity. Further testing of this protein is ongoing to determine its vaccine potential.