耐碳青霉烯类鲍曼不动杆菌噬菌体的分离鉴定及其生物学特性

目的通过分离耐碳青霉烯类鲍曼不动杆菌（CRAB）噬菌体,研究其生物学特性,为噬菌体治疗CRAB感染提供实验依据。方法利用噬菌斑法从医院污水中分离CRAB噬菌体,电子显微镜观察噬菌体的形态特征,提取其基因组行酶切电泳,SDS-PAGE分析噬菌体表面衣壳蛋白,测定噬菌体感染复数和耐受突变率,并观察其一步生长曲线。结果筛选出1株具有较宽裂解谱的无尾双链DNA的CRAB噬菌体,命名为噬菌体AB3。其基因组大小约35 kb,表面主要衣壳蛋白相对分子质量约为35×103。该噬菌体感染宿主菌的潜伏期为20 min,爆发期为60 min,裂解量为350,耐受突变率为2.5×10-10,对pH值耐受范围广,热稳...

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Published in上海交通大学学报（医学版） Vol. 32; no. 5; pp. 589 - 593
Main Author 张劼 罗永艾
Format Journal Article
LanguageChinese
Published 重庆市第三人民医院%重庆医科大学附属第一医院呼吸科,重庆,400016 2012
Subjects
噬菌体
生物学特性
鲍曼不动杆菌
生物学特性
鲍曼不动杆菌
噬菌体
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ISSN1674-8115
DOI10.3969/j.issn.1674-8115.2012.05.012

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Summary:目的通过分离耐碳青霉烯类鲍曼不动杆菌（CRAB）噬菌体,研究其生物学特性,为噬菌体治疗CRAB感染提供实验依据。方法利用噬菌斑法从医院污水中分离CRAB噬菌体,电子显微镜观察噬菌体的形态特征,提取其基因组行酶切电泳,SDS-PAGE分析噬菌体表面衣壳蛋白,测定噬菌体感染复数和耐受突变率,并观察其一步生长曲线。结果筛选出1株具有较宽裂解谱的无尾双链DNA的CRAB噬菌体,命名为噬菌体AB3。其基因组大小约35 kb,表面主要衣壳蛋白相对分子质量约为35×103。该噬菌体感染宿主菌的潜伏期为20 min,爆发期为60 min,裂解量为350,耐受突变率为2.5×10-10,对pH值耐受范围广,热稳定性好。结论噬菌体AB3具有较宽的噬菌谱,潜伏期短、裂解量大、耐受突变率低,对理化因素稳定性好,具有临床应用前景。
Bibliography:Acinetobacter baumannii; phages; biological characteristics
Objective To investigate the biological characteristics of phages of carbapenem-resistant Acinetobacter baumannii（CRAB）,and provide new strategy for controlling CRAB infection.Methods Phages of CRAB isolated from raw sewage of a hospital were identified using the plaque method.The morphology of phages was observed by electron microscopy.The genome of phages was extracted for electrophoresis.The structural proteins of phages were analysed by SDS-PAGE.The optimal multiplicity of infection,resistant mutation rate and one-step growth curve of phages were determined.Results One CRAB-specific and tailless phage with double-stranded DNA was isolated,and was named as phage AB3.The size of phage AB3 genome was about 35 kb,and the relative molecular mass of major coat protein was 35×103.The incubation and burst periods of phage AB3 were 20 min and 60 min respectively,the burst size was 350,and the mutation rate of phage resistance was 2.5×10-10.The phage AB3 wa
ISSN:1674-8115
DOI:10.3969/j.issn.1674-8115.2012.05.012