Exploration of novel predictive markers in rat plasma of the early stages of chronic renal failure

• Published in: Analytical and bioanalytical chemistry Vol. 406; no. 5; pp. 1365 - 1376
• Main Authors: Kobayashi, Toshihiro, Matsumura, Yuriko, Ozawa, Toshihiko, Yanai, Hiroyuki, Iwasawa, Atsuo, Kamachi, Toshiaki, Fujiwara, Kouichi, Tanaka, Noriaki, Kohno, Masahiro
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2014; Springer; Springer Nature B.V
• Subjects: Adenine; Adenosine - analogs & derivatives; Adenosine - blood; Analytical Chemistry; Anemia; Animals; Biochemistry; biomarkers; Biomarkers - blood; Blood; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatography; Chromatography, Liquid; Chronic kidney failure; Creatinine; Cresols - blood; Diagnosis; Diet; Discriminant analysis; Early Diagnosis; Food Science; Genetic aspects; Genetic markers; glycocholic acid; Glycocholic Acid - blood; Hemodialysis; Indican - blood; Kidney diseases; Kidney Failure, Chronic - blood; Kidney Failure, Chronic - chemically induced; Kidney Failure, Chronic - diagnosis; Laboratory Medicine; Liquid chromatography; Lysophospholipids - blood; Male; Mass spectrometry; Metabolites; Metabolomics; Monitoring/Environmental Analysis; Multivariate Analysis; Plasma; Proteins; Rats; Rats, Sprague-Dawley; renal failure; Renal function; Research Paper; Scientific imaging; Sulfates; Sulfuric Acid Esters - blood; Tandem Mass Spectrometry; Toxins; Japan; United States > US; LC/MS; Metabolomics; Animal model; Chronic renal failure
• ISSN: 1618-2642; 1618-2650; 1618-2650
• DOI: 10.1007/s00216-013-7472-2

To identify blood markers for early stages of chronic kidney disease (CKD), blood samples were collected from rats with adenine-induced CKD over 28 days. Plasma samples were subjected to metabolomic profiling by liquid chromatography-mass spectrometry, followed by multivariate analyses. In addition to already-identified uremic toxins, we found that plasma concentrations of N6-succinyl adenosine, lysophosphatidylethanolamine 20:4, and glycocholic acid were altered, and that these changes during early CKD were more sensitive markers than creatinine concentration, a universal indicator of renal dysfunction. Moreover, the increase in plasma indoxyl sulfate concentration occurred earlier than increases in phenyl sulfate and p -cresol sulfate. These novel metabolites may serve as biomarkers in identifying early stage CKD.