miRNA-140-5p在人骨髓间充质干细胞成脂分化中的表达及其靶基因的预测

目的筛选出人骨髓间充质干细胞（h MSCs）成脂分化中具有潜在价值的mi RNA及其靶基因,为人类成骨细胞与脂肪细胞分化平衡调控机制的深入研究提供有力实验依据。方法收集h MSCs成脂分化过程中的0、7、14、21 d的细胞样品,应用mi RNA芯片和转录组测序（m RNA-seq）技术,分析4个点细胞中mi RNA和m RNA的表达水平,并将mi RNA与m RNA的表达趋势进行关联分析,聚焦具有研究价值的mi RNA与m RNA相互调控关系,同时,采用Target Scan、Pic Tar和mi Randa等数据库进行靶基因的预测。结果发现mi R-140-5p在成脂分化中表达量呈逐渐下降...

Full description

Saved in:
Bibliographic Details
Published in南方医科大学学报 Vol. 37; no. 2; pp. 199 - 203
Main Author 汪涛 颜瑞巧 曹俊 曹玲玲 张铉浦 李兴暖 吴萍 周小鸥 吴建芳 许晓源
Format Journal Article
LanguageChinese
Published 江西省九江学院基础医学院病原生物学教研室,江西九江332000%江西省九江学院附属医院临床技能中心,江西九江,332000%江西省系统生物医学重点实验室,江西九江,332000%江西省南昌大学附属九江医院内分泌科,江西九江,332000 2017
江西省系统生物医学重点实验室,江西九江332000
Subjects
mi
RNA-140-5p
人骨髓间充质干细胞
成脂分化
白血病抑制因子受体
成脂分化
leukemia inhibitory factor receptor
白血病抑制因子受体
adipogenic differentiation
miRNA-140-Sp
miRNA-140-5p
人骨髓间充质干细胞
human bone marrow mesenchymal stem cells
Online AccessGet full text
ISSN1673-4254
DOI10.3969/j.issn.1673-4254.2017.02.09

Cover

More Information
Summary:目的筛选出人骨髓间充质干细胞（h MSCs）成脂分化中具有潜在价值的mi RNA及其靶基因,为人类成骨细胞与脂肪细胞分化平衡调控机制的深入研究提供有力实验依据。方法收集h MSCs成脂分化过程中的0、7、14、21 d的细胞样品,应用mi RNA芯片和转录组测序（m RNA-seq）技术,分析4个点细胞中mi RNA和m RNA的表达水平,并将mi RNA与m RNA的表达趋势进行关联分析,聚焦具有研究价值的mi RNA与m RNA相互调控关系,同时,采用Target Scan、Pic Tar和mi Randa等数据库进行靶基因的预测。结果发现mi R-140-5p在成脂分化中表达量呈逐渐下降的趋势,而白血病抑制因子受体（LIFR）表达量呈逐渐升高趋势,二者呈现负调控的对应关系。同时经3个靶基因数据库预测,LIFR为mi R-140-5p共有的靶基因。结论 mi RNA-140-5p可能与人骨髓间充质干细胞的成脂分化密切相关,且通过调控其靶基因LIFR的表达而参与成脂分化。
Bibliography:44-1627/R
human bone marrow mesenchymal stem cells; adipogenic differentiation; miRNA-140-5p; leukemia inhibitory factor receptor
WANG Tao1, 2, YAN Ruiqiao3, CAO Jun1, CAO Lingling4, ZHANG Xuanpu4, LI Xingnuan1, WU Ping1, ZHOU Xiaoou1, 2, WU Jianfang1, 2, XU Xiaoyuan1 （1.Key Laboratory of fiangxi Province for Systems Biomedicine, Jiujiang 332000, China; 2Department of Pathogenic Biology, School of Basic Medical Sciences, Jiujiang University, Jiujiang 332000, China; 3.Clinical Skills Center, Affiliated Hospital of Jiujiang University, Jiujiang 332000, China; 4Department of Endocrinology, Jiujiang Hospital Affiliated to Nanchang University, Jiujiang 332000, China）
Objective To screen the differentially expressed miRNAs and their target genes in adipogenic differentiation of human bone marrow mesenchymal stem cells （hMSCs） to better understand the mechanism for regulating the balance between osteoblast and adipocyte differentiation. Methods Cultured hMSCs were induced for adipogenic differentiation, and at 0, 7, 14
ISSN:1673-4254
DOI:10.3969/j.issn.1673-4254.2017.02.09