三株生防酵母菌对Botrytis cinerea的抑菌作用研究

针对三株生防酵母菌Cryptococcus albidus 63（Ca63）、Cryptococcus albidus 64（Ca64）、Candida parapsilosis yett1006对番茄灰霉病病原菌B. cinerea t08016b的抑菌作用进行研究。三株生防酵母菌与B. cinerea t08016b的对峙培养过程中，未产生明显抑菌圈，均可减弱B. cinerea t08016b生长势；几丁质酶和β-1，3-葡聚糖酶活性测定结果表明， t08016b并无诱导三株酵母菌分泌酶活显著增加（P＞ 0.05）；研究酵母菌对B. cinerea t08016b菌丝生长和孢子萌发率影响...

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Published in东北农业大学学报 Vol. 45; no. 7; pp. 54 - 60
Main Author 王傲雪 关鑫 张俊峰 张珍珠 王瑞虎 陈秀玲
Format Journal Article
LanguageChinese
Published 东北农业大学生命科学学院，哈尔滨 150030%东北农业大学园艺学院,哈尔滨,150030%东北农业大学生命科学学院,哈尔滨,150030 2014
东北农业大学园艺学院，哈尔滨 150030
Subjects
β-1,3-葡聚糖酶酶活
几丁质酶酶活
孢子萌发率
生防酵母菌
菌丝生长
β-1,3-葡聚糖酶酶活
chitinase activity
spore germination rate
biocontrol yeast
孢子萌发率
生防酵母菌
几丁质酶酶活
菌丝生长
mycelium growth
β-1,3-glucanase activity
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ISSN1005-9369

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Summary:针对三株生防酵母菌Cryptococcus albidus 63（Ca63）、Cryptococcus albidus 64（Ca64）、Candida parapsilosis yett1006对番茄灰霉病病原菌B. cinerea t08016b的抑菌作用进行研究。三株生防酵母菌与B. cinerea t08016b的对峙培养过程中，未产生明显抑菌圈，均可减弱B. cinerea t08016b生长势；几丁质酶和β-1，3-葡聚糖酶活性测定结果表明， t08016b并无诱导三株酵母菌分泌酶活显著增加（P＞ 0.05）；研究酵母菌对B. cinerea t08016b菌丝生长和孢子萌发率影响发现，C.albidus 63、C.albidus 64和C.parapsilosis yett1006对菌丝生长量的抑制率分别为88.57%、90.66%和85.65%，高浓度菌液对孢子萌发有显著抑制效果，1 × 10^8 cfu·ML^-1菌体活细胞能完全抑制病原菌孢子萌发，菌体活细胞浓度越低抑制效果越不明显，无菌滤液和灭活的菌体活细胞对病原孢子的萌发无显著抑制效果。结果表明，生防酵母菌主要通过竞争有限的生长空间和营养条件，而非通过分泌抑菌物质实现拮抗作用。
Bibliography:In this study, the antifungal activities of biocontrol yeasts Cryptococcus albidus 63（Ca63）, Cryptococcus albidus 64 （Ca64） and Candida parapsilosis yett1006 against Botrytis cinerea were investigated. The dual culture between three biocontrol yeasts and B. cinerea t08016b results showed that no obvious inhibiting zone appeared, but the pathogen growed more weakly than the control. The results of chitinase andβ-1, 3-glucanase activities assay showed that the levels of the two enzymes activities did not change significantly （P＆gt;0.05） when the three biocontrol yeasts cultured with pathogen. The inhibition rates of three biocontrol yeast strains on mycelium growth were 88.57%, 90.66% and 85.65%, respectively. High concentrations of biocontrol yeasts had a strong inhibitory effect on the spore germination of B. cinerea t08016b. Especial y, 1 × 10^8 cfu·mL^-1 biocontrol yeasts almost stopped the spore germination of B. cinerea t08016b. Heat-inactived yeast suspension and culture supernatant had no significant in
ISSN:1005-9369