Direct ex vivo analysis of allergen-specific CD4+ T cells

• Published in: Journal of allergy and clinical immunology Vol. 125; no. 6; pp. 1407 - 1409.e1
• Main Authors: Kwok, William W., Roti, Michelle, DeLong, Jonathan H., Tan, Venus, Wambre, Erik, James, Eddie A., Robinson, David
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.06.2010; Elsevier; Elsevier Limited
• Subjects: Allergies; Allergy and Immunology; Animals; Biological and medical sciences; Cats; CD4-Positive T-Lymphocytes - immunology; CD4-Positive T-Lymphocytes - metabolism; CD4-Positive T-Lymphocytes - pathology; Cell Separation; Cells, Cultured; Cytokines; Epitope Mapping; Epitopes, T-Lymphocyte - chemistry; Epitopes, T-Lymphocyte - immunology; Epitopes, T-Lymphocyte - metabolism; Flow Cytometry; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Genotype & phenotype; Glycoproteins - chemistry; Glycoproteins - immunology; Glycoproteins - metabolism; Histocompatibility Antigens Class II - immunology; Histocompatibility Antigens Class II - metabolism; Humans; Hypersensitivity - diagnosis; Hypersensitivity - immunology; Immunopathology; Immunophenotyping; Influenza; Lymphocytes; Medical sciences; Peptide Fragments - chemistry; Peptide Fragments - immunology; Peptide Fragments - metabolism; Peptides; Protein Binding; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; Studies; Allergy; Immunopathology; Immunology; CD4 T lymphocyte; Ex vivo; Allergen
• ISSN: 0091-6749; 1097-6825; 1097-6825
• DOI: 10.1016/j.jaci.2010.03.037

Influenza-specific T cells were CD45RO and CD28-positive, but were CXCR3-positive, CCR4-negative, and CRTH2-negative. [...]allergen-specific TÂ cells show a distinct phenotype compared with influenza-specific T cells and the total CD4+ T-cell population. [...]TGEM facilitates rapid identification of T-cell epitopes.6 Third, enrichment methods allow direct ex vivo detection of antigen-specific T cells.7 This study applied these methods to visualize and characterize Fel d 1-specific T cells in subjects with allergy.