基质辅助激光解吸电离飞行时间质谱快速鉴定蜡样芽胞杆菌研究与应用
建立基于基质辅助激光解吸电离飞行时间质谱（MALDI—TOF—MS）技术针对食源性蜡样芽胞杆菌快速鉴定方法。以蜡样芽胞杆菌模式菌株为研究对象，优化该菌样品处理方法，并选取17株蜡样芽胞杆菌标准菌株补充MALDI-TOF—MS数据库，探讨方法灵敏度、稳定性和准确性。结果表明，蜡样芽胞杆菌最佳样品处理方法是甲酸提取法，数据库补充可提高该菌鉴定可信度，MALDI-TOF—MS鉴定方法准确度高、稳定性好，灵敏度为104cfu并通过聚类分析分型。研究确定的MALDI—TOF—MS鉴定方法准确快速，可为食源性蜡样芽胞杆菌快速鉴定和分型提供技术支持。...
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| Published in | 东北农业大学学报 Vol. 47; no. 12; pp. 56 - 64 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
东北农业大学食品学院,哈尔滨,150030%中国检验检疫科学研究院农产品安全研究中心,北京,100176
2016
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1005-9369 |
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| Summary: | 建立基于基质辅助激光解吸电离飞行时间质谱（MALDI—TOF—MS）技术针对食源性蜡样芽胞杆菌快速鉴定方法。以蜡样芽胞杆菌模式菌株为研究对象，优化该菌样品处理方法，并选取17株蜡样芽胞杆菌标准菌株补充MALDI-TOF—MS数据库，探讨方法灵敏度、稳定性和准确性。结果表明，蜡样芽胞杆菌最佳样品处理方法是甲酸提取法，数据库补充可提高该菌鉴定可信度，MALDI-TOF—MS鉴定方法准确度高、稳定性好，灵敏度为104cfu并通过聚类分析分型。研究确定的MALDI—TOF—MS鉴定方法准确快速，可为食源性蜡样芽胞杆菌快速鉴定和分型提供技术支持。 |
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| Bibliography: | Identification method for Bacillus cereus in food by matrix assisted laser desorption/ ionization time of flight mass spectrometry （MALDI-TOF-MS） was established. This study optimized sample handling methods, focusing on Bacillus cereus type strain, and 17 standard strains of Bacillus cereus were used to supplement a MALDI-TOF-MS database, the sensitivity, stability, accuracy of the method were evaluated. The results showed that the optimal sample handling method of Bacillus cereus was formic acid treatment method, the credibility of identification results of Bacillus cereus was improved by supplementing the database. Based on these conditions, the MALDI-TOF-MS method showed excellent accuracy, stability and appropriate sensitivity （10^4 cfu）. Further typing was achieved by cluster analysis. The identification method for Bacillus cereus by MALDI-TOF-MS is accurate, rapid, and it could provJde a technical support for identification and typing of Bacillus cereus in food. 23-1391/S Bacillus cereus; MALDI-TOF-MS; i |
| ISSN: | 1005-9369 |