猪沙门氏菌的分离鉴定及其耐药性分析

对哈尔滨地区规模化猪场沙门氏菌进行分离鉴定，分析其对β-内酰胺类药物的药敏试验结果及耐药情况。采用病原菌的形态学观察、生化鉴定和PCR鉴定，从600份哈尔滨地区规模化猪场猪直肠粪便中分离出沙门氏菌，运用Kirby-Bauer法检测分离菌对10种β-内酰胺类药物的敏感性。结果表明，在哈尔滨地区十市县的猪群中，沙门氏菌的总检出率为5.83%（35/600）；不同市县沙门氏菌的总分离率介于3.3%-18.3%；沙门氏菌的分离率与猪日龄有一定关系；对临床常用抗菌药物均表现出较高的耐药性，对氨苄西林100%耐药，对头孢他啶80%耐药，对头孢西丁、哌拉西林、头孢噻肟、头孢吡肟、氨曲南存在不同程度耐药，对碳...

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Published in东北农业大学学报 Vol. 45; no. 8; pp. 49 - 54
Main Author 加春生 毛泽明 王晓楠 王俊峰 王龙 陈爽 葛兰云 徐世文
Format Journal Article
LanguageChinese
Published 黑龙江农业工程职业学院，哈尔滨 150088%哈尔滨鑫广丰牧业有限公司,哈尔滨,150030%黑龙江农业工程职业学院,哈尔滨,150088%东北农业大学动物医学学院,哈尔滨,150030 2014
东北农业大学动物医学学院，哈尔滨 150030
Subjects
分离鉴定
沙门氏菌
猪
耐药性
沙门氏菌
isolation and identification
耐药性
猪
Salmonela
resistance
分离鉴定
pig
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ISSN1005-9369

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Summary:对哈尔滨地区规模化猪场沙门氏菌进行分离鉴定，分析其对β-内酰胺类药物的药敏试验结果及耐药情况。采用病原菌的形态学观察、生化鉴定和PCR鉴定，从600份哈尔滨地区规模化猪场猪直肠粪便中分离出沙门氏菌，运用Kirby-Bauer法检测分离菌对10种β-内酰胺类药物的敏感性。结果表明，在哈尔滨地区十市县的猪群中，沙门氏菌的总检出率为5.83%（35/600）；不同市县沙门氏菌的总分离率介于3.3%-18.3%；沙门氏菌的分离率与猪日龄有一定关系；对临床常用抗菌药物均表现出较高的耐药性，对氨苄西林100%耐药，对头孢他啶80%耐药，对头孢西丁、哌拉西林、头孢噻肟、头孢吡肟、氨曲南存在不同程度耐药，对碳青霉烯类敏感。结果表明，哈尔滨地区猪场存在不同程度的沙门氏菌感染，耐药现象严重。
Bibliography:Serological identification of Salmonella in the large-scale pig farms of Haerbin, and analyzed the different Salmonella antimicrobial susceptibility testresults and drug resistance.Methods Up to 35 Salmonella strains were isolated from 600 feces in the rectal of pigs by morphological observation and biochemical system. The sensitivity tests of 35 strains to 10 Beta lactam antibacterials were performed by Krby-Bauer method.These microorganisms were further confirmed by correspond-ing specific bio-chemistry assay and polymerase chain reaction （PCR）. The reasults showed that the overall detection rate of Salmonella was 5.83%and the total rate of separation was from 3.3%to 18.3%in the different citys of Harbin. The resistant rate of 35 strains to ampicillin, ceftazidime, cefoxitin, piperacillin, cefotaxime, cefepime and aztreonam was 100%, 80%, 17%, 71%, 66%, 6 3% and 29%respectively. However, they were still sensitive to ertapenem,meropenem and imipenem. The results showed that Salmonella infections exist in pig
ISSN:1005-9369