阿司匹林联合辛伐他汀对成骨细胞增殖及分化的影响

目的探索阿司匹林（aspirin,ASP）联合辛伐他汀（simvastatin,SIM）对成骨细胞增殖和分化的影响,为临床上两者联合使用的可行性提供细胞学基础。方法获取SD大鼠成骨细胞,随机分为对照组（CON）、ASP组、SIM组和ASP-SIM组等4组,培养基中分别添加安慰剂、ASP、SIM和ASP联合SIM干预,培养7 d后,通过MTT法检测细胞的增殖情况,碱性磷酸酶（alkaline phosphatase,ALP）及茜素红染色观察细胞的功能状态,蛋白电泳观察骨钙素（osteocalcin,OCN）及Runx2蛋白的表达情况。结果 ASP及SIM单独作用于成骨细胞时,都可以明显促进成骨细...

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Bibliographic Details
Published in中国骨质疏松杂志 Vol. 24; no. 2; pp. 170 - 173
Main Author 周皖舒;陶周善
Format Journal Article
LanguageChinese
Published 皖南医学院第二附属医院老年病科,安徽 芜湖241000 %皖南医学院第一附属医院,弋矶山医院创伤骨科,安徽 芜湖241000 2018
Subjects
阿司匹林;辛伐他汀;成骨细胞;增殖;分化;骨质疏松
骨质疏松
Osteoporosis
Aspirin
增殖
辛伐他汀
阿司匹林
Simvastatin
成骨细胞
分化
Proliferation
Differentiation
Osteoblasts
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ISSN1006-7108
DOI10.3969/j.issn.1006-7108.2018.02.007

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Summary:目的探索阿司匹林（aspirin,ASP）联合辛伐他汀（simvastatin,SIM）对成骨细胞增殖和分化的影响,为临床上两者联合使用的可行性提供细胞学基础。方法获取SD大鼠成骨细胞,随机分为对照组（CON）、ASP组、SIM组和ASP-SIM组等4组,培养基中分别添加安慰剂、ASP、SIM和ASP联合SIM干预,培养7 d后,通过MTT法检测细胞的增殖情况,碱性磷酸酶（alkaline phosphatase,ALP）及茜素红染色观察细胞的功能状态,蛋白电泳观察骨钙素（osteocalcin,OCN）及Runx2蛋白的表达情况。结果 ASP及SIM单独作用于成骨细胞时,都可以明显促进成骨细胞的增殖,增加ALP活性及矿化能力,同时明显促进OCN及Runx2蛋白的表达;联合使用效果明显优于单独使用,比较差异有统计学意义（P〈0.05）。结论 ASP及SIM都可以明显促进成骨细胞增殖和分化,且联合使用效果更佳。
Bibliography:ZHOU Wanshu1, TAO Zhoushan2. （1. Department of Geriatrics, Second Affiliated Hospital of Wannan Medical College, Wuhu, Anhu 241000; 2. Department of Orthopedics, First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu, Anhu 241000 China）
Aspirin ; Simvastatin; Osteoblasts ; Proliferation; Differentiation; Osteoporosis
Objective To explore the effect of Aspirin（ ASP） combined with Simvastatin（ SIM） on osteoblast proliferation and differentiation,and to provide a cytological basis for the feasibility of combination therapy. Methods SD rat osteoblasts were randomly divided into 4 groups： control group,ASP group,SIM group and ASP-SIM group. The cell culture was supplemented with placebo,ASP,SIM and ASP combined with SIM. After 7 days of culture,the proliferation of cells was detected by MTT assay. The functional status of cells was observed by ALP and alizarin red staining. The expression of OCN and Runx2 protein were observed by protein electrophoresis. Results ASP and SIM could significantly
ISSN:1006-7108
DOI:10.3969/j.issn.1006-7108.2018.02.007