骨形成蛋白联合雷奈酸锶对成骨细胞增殖和分化的影响

目的探索骨形成蛋白（bone morphogenetic protein,BMP）联合雷奈酸锶（strontium ranelate,SR）对成骨细胞增殖和分化的影响,为临床上两者联合使用可行性提供细胞学基础。方法获取SD大鼠成骨细胞,随机分为对照组、SR组、BMP组和BMP-SR组等4组,培养基中分别添加安慰剂、SR、BMP-2和SR联合BMP-2干预,培养12 d后,通过MTT法检测细胞的增殖情况,碱性磷酸酶（alkaline phosphatase,ALP）及茜素红染色观察细胞的功能状态,蛋白电泳观察骨钙素（osteocalcin,OCN）及Runx2蛋白的表达情况。结果 SR及BMP-...

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Bibliographic Details
Published in中国骨质疏松杂志 Vol. 24; no. 2; pp. 165 - 169
Main Author 陶周善;周皖舒;江云云;吴兴净;张欣;杨民;谢加兵;徐祝军
Format Journal Article
LanguageChinese
Published 皖南医学院第一附属医院,弋矶山医院创伤骨科,安徽 芜湖241000%皖南医学院第二附属医院老年病科,安徽 芜湖,241000 2018
Subjects
骨形成蛋白-2;雷奈酸锶;成骨细胞;增殖;分化;骨质疏松
骨形成蛋白-2
骨质疏松
Bone morphogenetic protein-2
Osteoporosis
增殖
成骨细胞
Strontium ranelate
分化
Proliferation
Differentiation
Osteoblasts
雷奈酸锶
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ISSN1006-7108
DOI10.3969/j.issn.1006-7108.2018.02.006

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Summary:目的探索骨形成蛋白（bone morphogenetic protein,BMP）联合雷奈酸锶（strontium ranelate,SR）对成骨细胞增殖和分化的影响,为临床上两者联合使用可行性提供细胞学基础。方法获取SD大鼠成骨细胞,随机分为对照组、SR组、BMP组和BMP-SR组等4组,培养基中分别添加安慰剂、SR、BMP-2和SR联合BMP-2干预,培养12 d后,通过MTT法检测细胞的增殖情况,碱性磷酸酶（alkaline phosphatase,ALP）及茜素红染色观察细胞的功能状态,蛋白电泳观察骨钙素（osteocalcin,OCN）及Runx2蛋白的表达情况。结果 SR及BMP-2单独作用于成骨细胞时,都可以明显促进成骨细胞的增殖,增加ALP活性及矿化能力,同时明显促进OCN及Runx2蛋白的表达;联合使用效果明显优于单独使用,比较差异有统计学意义（P〈0.05）。结论 BMP-2及SR都可以明显促进成骨细胞增殖和分化,且联合使用效果更佳。
Bibliography:Bone morphogenetic protein-2 ; Strontium ranelate ; Osteoblasts ; Proliferation ; Differentiation ; Osteoporosis
Objective To explore the effect of bone morphogenetic protein（ BMP） combined with strontium ranelate（ SR） on osteoblast proliferation and differentiation,and to provide a cytological basis for the feasibility of combination therapy. Methods Osteoblasts obtained from SD rat were randomly divided into 4 groups： control group,SR group,BMP group and BMP-SR group.The cell culture was supplemented with placebo,SR,BMP-2 and SR combined with BMP. After 12 days of culture,the proliferation of cells was detected by MTT assay. The functional status of cells was observed by ALP and alizarin red staining. The expression of OCN and Runx2 protein was observed by protein electrophoresis. Results SR and BMP-2 could significantly promote the proliferation of osteoblasts,increase the activity and mineralization ability of osteoblasts,and promote the expression of OCN and Runx2 protein. The effect of combination was si
ISSN:1006-7108
DOI:10.3969/j.issn.1006-7108.2018.02.006