Ghrelin对糖尿病大鼠心肌动作电位和瞬时外向钾电流的影响

目的 探讨外源性ghrelin对糖尿病心肌病（DCM）大鼠心肌动作电位及瞬时外向钾电流（Ito）的影响。方法雄性SD大鼠腹腔注射链脲佐菌素（60mg/kg）诱导Ⅰ型DCM模型,并采用全细胞膜片钳技术观察ghrelin对糖尿病大鼠心室肌细胞动作电位及瞬时外向钾电流的影响。结果 与DCM组比较,10^-7 mmol/L ghrelin明显延长DCM大鼠心室肌细胞5%动作电位时程（APD50）[（12.49±2.32）%;n=7,P=0.037]及APD90[（26.29±5.13）%;n=7,P=0.006];并明显抑制Ito电流峰值,抑制率[（23.14±3.07）%;n=9,P=0.021]。...

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Published in西安交通大学学报（医学版） Vol. 38; no. 5; pp. 661 - 664
Main Author 冯丹阳 孙强 陈莉娜 赵正杭 周筠
Format Journal Article
LanguageChinese
Published 西安市电力中心医院药剂科,陕西西安 710032%西安交通大学医学部基础医学院药理学系,陕西西安,710061 2017
西安交通大学医学部基础医学院药理学系,陕西西安 710061
Subjects
ghrelin
动作电位
瞬时外向钾电流
糖尿病心肌病
ghrelin
糖尿病心肌病
动作电位
transient outside potassium current
瞬时外向钾电流
diabetic cardiomyopathy
action potential
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ISSN1671-8259
DOI10.7652/jdyxb201705007

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Summary:目的 探讨外源性ghrelin对糖尿病心肌病（DCM）大鼠心肌动作电位及瞬时外向钾电流（Ito）的影响。方法雄性SD大鼠腹腔注射链脲佐菌素（60mg/kg）诱导Ⅰ型DCM模型,并采用全细胞膜片钳技术观察ghrelin对糖尿病大鼠心室肌细胞动作电位及瞬时外向钾电流的影响。结果 与DCM组比较,10^-7 mmol/L ghrelin明显延长DCM大鼠心室肌细胞5%动作电位时程（APD50）[（12.49±2.32）%;n=7,P=0.037]及APD90[（26.29±5.13）%;n=7,P=0.006];并明显抑制Ito电流峰值,抑制率[（23.14±3.07）%;n=9,P=0.021]。结论 外源性ghrelin抑制Ito电流而延长DCM大鼠心肌动作电位时程,参与糖尿病大鼠心脏电生理的重构。
Bibliography:Objective To study the effects of ghrelin on action potential （AP） and transient outside K^＋ current （I,o） in ventricular myocytes of diabetic cardiomyopathy （DCM） rats. Methods A rat model of DCM was established by single intraperitoneal injection of streptozotocin （STZ, 60 mg/kg）, and whole cell patch-clamp technique was used to record the effects of 10.7 mmol/L ghrelin on AP and /to current density in the DCM rat ventricular myocytes. Results Compared to DCM group, Ghrelin of 10.7 mmol/L could significantly prolong APDs0[（12.49±2.32）%; n=7, P=0.037] and APD90[（26.29±5.13）%; n=7, P=0.006] and decrease /to current peak value [（23. 14± 3.07）% ; n = 9, P= 0. 021] in DCM rat ventricular myocytes. Conclusion Exogenous ghrelin is involved in the electrophysiological reconstruction of the heart of diabetic rats by decreasing /to current and prolonging APD in STZ-induced DCM rat ventricular myocytes.
FENG Da n-yang1,2, SUN Qiang1, CHEN Li-na1, ZHAO Zheng-hang1, ZHOU Jun1 （1.Department of Pharmacology, School of Basi
ISSN:1671-8259
DOI:10.7652/jdyxb201705007