低聚壳聚糖与α-丙氨酸/天冬酰胺的美拉德反应及其衍生物的抗氧化性能研究

研究低聚壳聚糖（COS）与α-丙氨酸/天冬酰胺的美拉德反应,考察了两个体系（低聚壳聚糖的羰基与氨基的物质量比均为1∶1）的pH、吸光度和荧光值的变化。醇沉法提取低聚壳聚糖衍生物CA和CN。对两种衍生物进行红外表征和分子量测定,并研究其对超氧阳离子O2-.、DPPH自由基的清除能力以及还原能力。结果显示：抗氧化能力强弱次序为CA〉CN〉COS,即美拉德反应后低聚壳聚糖衍生物抗氧化能力得到显著提高,且CA的抗氧化活性优于CN,表明与小分子氨基酸进行美拉德反应制得的壳聚糖衍生物具有更好的抗氧化性。...

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Published in天然产物研究与开发 Vol. 24; no. 4; pp. 450 - 453
Main Author 孙涛 朱云 谢晶 薛斌 毛芳
Format Journal Article
LanguageChinese
Published 上海海洋大学海洋科学研究院,上海201306%上海海洋大学食品学院 2012
上海海洋大学食品学院
Subjects
α-丙氨酸
低聚壳聚糖
天冬酰胺
抗氧化性能
美拉德反应
美拉德反应
低聚壳聚糖
α-丙氨酸
天冬酰胺
抗氧化性能
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ISSN1001-6880
DOI10.3969/j.issn.1001-6880.2012.04.006

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Summary:研究低聚壳聚糖（COS）与α-丙氨酸/天冬酰胺的美拉德反应,考察了两个体系（低聚壳聚糖的羰基与氨基的物质量比均为1∶1）的pH、吸光度和荧光值的变化。醇沉法提取低聚壳聚糖衍生物CA和CN。对两种衍生物进行红外表征和分子量测定,并研究其对超氧阳离子O2-.、DPPH自由基的清除能力以及还原能力。结果显示：抗氧化能力强弱次序为CA〉CN〉COS,即美拉德反应后低聚壳聚糖衍生物抗氧化能力得到显著提高,且CA的抗氧化活性优于CN,表明与小分子氨基酸进行美拉德反应制得的壳聚糖衍生物具有更好的抗氧化性。
Bibliography:SUN Tao1,2＊,ZHU Yun1,XIE Jing1,XUE Bin1,MAO Fang1 1College of Food Science and Technology,Shanghai Ocean University;2Institute of Marine Science,Shanghai Ocean University,Shanghai 201306,China
chitosan oligosaccharide; α-alanine; asparagines; Maillard reaction; antioxidant activity
Two kinds of the chitosan oligosaccharide derivatives were prepared through Maillard reaction by heating chitosan oligosaccharide and α-alanine / asparagine（the ratio of carbonyl group and amino group was 1∶ 1）.The pH,absorbance and fluorescence were determined during the reaction.The chitosan oligosaccharide derivatives were characterized by FTIR and their molecular weight was determined by GPC.Their antioxidant activity was investigated by employing various established in vitro systems,such as superoxide,1,1-diphenyl-2-picrylhydrazyl（DPPH） radicals scavenging and reducing power.The results showed the chitosan oligosaccharide derivatives had strong antioxidant activity compared to chitosan oligosaccharide.The antioxidant activity wa
ISSN:1001-6880
DOI:10.3969/j.issn.1001-6880.2012.04.006