FGF-21促进HepG2细胞摄取葡萄糖研究

肝脏是代谢的中枢性器官,在糖脂代谢中扮演重要角色。FGF-21是近年来发现的一种治疗糖尿病新型药物,研究其对肝脏糖代谢影响及机制将为FGF-21成药性提供理论依据。以Hep G2细胞为肝细胞模型,探究FGF-21对Hep G2细胞葡萄糖吸收影响及作用机制。FGF-21处理Hep G2细胞,采用葡萄糖氧化酶-过氧化物酶（GOD-POD）法检测细胞对葡萄糖摄取情况,并检查胰岛素与FGF-21协同作用,蒽酮法检测细胞内糖原含量,半定量和实时荧光定量PCR检测FGF-21对葡萄糖转运蛋白（GLUTs）m RNA表达影响。结果表明,FGF-21可促进Hep G2细胞摄取葡萄糖,与胰岛素具有一定协同作用,...

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Published in东北农业大学学报 Vol. 47; no. 5; pp. 36 - 43
Main Author 刘铭瑶 王文飞 侯玉婷 任桂萍 吴云舟 李德山
Format Journal Article
LanguageChinese
Published 东北农业大学生命科学学院,哈尔滨,150030%长春卫尔赛生物药业有限公司,长春,130616 2016
Subjects
FGF-21
HepG2
糖代谢
糖尿病
HepG2 cell line
HepG2
糖代谢
FGF-21
糖尿病
diabetes
glucose metabolism
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ISSN1005-9369

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Summary:肝脏是代谢的中枢性器官,在糖脂代谢中扮演重要角色。FGF-21是近年来发现的一种治疗糖尿病新型药物,研究其对肝脏糖代谢影响及机制将为FGF-21成药性提供理论依据。以Hep G2细胞为肝细胞模型,探究FGF-21对Hep G2细胞葡萄糖吸收影响及作用机制。FGF-21处理Hep G2细胞,采用葡萄糖氧化酶-过氧化物酶（GOD-POD）法检测细胞对葡萄糖摄取情况,并检查胰岛素与FGF-21协同作用,蒽酮法检测细胞内糖原含量,半定量和实时荧光定量PCR检测FGF-21对葡萄糖转运蛋白（GLUTs）m RNA表达影响。结果表明,FGF-21可促进Hep G2细胞摄取葡萄糖,与胰岛素具有一定协同作用,增加糖原合成。半定量PCR结果显示在FGF-21作用下,仅GLUT1m RNA表达有所增加。实时荧光定量PCR检测FGF-21作用时间对GLUT1 m RNA表达量影响,发现FGF-21作用6 h时GLUT1 m RNA表达量倍数增加最大。说明FGF-21可通过增加GLUT1 m RNA表达促进Hep G2细胞消耗葡萄糖,参与糖原合成。
Bibliography:23-1391/S
LIU Mingyao, WANG Wenfei, HOU Yuting, REN Gulping, WU Yunzhou, LI Deshan（1. School of Life Sciences, Northeast Agricultural University, Harbin 150030, China; 2. Changchun Weresai Biotic Pharma- ceutical Co. Ltd, Changchun 130616, China）
diabetes; glucose metabolism; FGF-21; HepG2 cell line
Liver is a metabolic center, plays an important role in regulating glucose and lipid metabolism. FGF- 21 is an important candidate drug to treat diabetes; so to study the effect and mechanism of its effects on regulation of the glucose metabolism in liver is very important for drug research. We chose Hep G2 cells as a model to study the effects of FGF-21 on glucose uptake in liver and the mechanism of its action. After treated by FGF-21, the glucose uptake by the Hep G2 cells was detected by the method of glucose oxidizes · peroxides（GOD-POD）; glycogen synthesis was examined by anthrone method, the synergy between insulin and FGF-21 was evaluated. With the purpose to study the mechanism of FGF- 21 on glucose uptake,
ISSN:1005-9369