RGD肽修饰壳聚糖作为种植体表面基因载体的研究

目的： 探索改善钛种植体表面处理的新方法，提高种植体植入后成骨效率。方法 将RGD肽与壳聚糖（CS）通过酰化反应发生偶联形成RGD-CS，采用复凝聚法制备RGD-CS/pDNA复合体，并将RGD-CS/pDNA复合体接枝到经物理、生化处理后的纯钛表面。采用红外光谱仪和元素分析仪对RGD-CS进行化学结构的表征检测，凝胶电泳阻滞试验结合原子力显微镜观察RGD-CS对质粒的包裹情况及RGD-CS/pDNA复合体的形态，EB染色法检测钛片表面RGD-CS/pDNA复合体的接枝效果。结果 红外光谱检测结合元素分析表明，CS和RGD肽偶联成功；凝胶电泳阻滞试验结合原子力显微镜观察表明，在N/P≥2时，R...

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Published in华西口腔医学杂志 Vol. 32; no. 4; pp. 336 - 340
Main Author 张迪 刘长虹 章锦才 蔡德鸿 杨晓喻 李世轶 钟惠兰
Format Journal Article
LanguageChinese
Published 南方医科大学附属口腔医院·广东省口腔医院种植科，广州 510280%南方医科大学附属珠江医院内分泌科,广州,510282 2014
Subjects
RGD肽
壳聚糖
种植体
钛
chitosan
壳聚糖
种植体
RGD peptide
titanium
RGD肽
钛
implant
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ISSN1000-1182
DOI10.7518/hxkq.2014.04.005

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Summary:目的： 探索改善钛种植体表面处理的新方法，提高种植体植入后成骨效率。方法 将RGD肽与壳聚糖（CS）通过酰化反应发生偶联形成RGD-CS，采用复凝聚法制备RGD-CS/pDNA复合体，并将RGD-CS/pDNA复合体接枝到经物理、生化处理后的纯钛表面。采用红外光谱仪和元素分析仪对RGD-CS进行化学结构的表征检测，凝胶电泳阻滞试验结合原子力显微镜观察RGD-CS对质粒的包裹情况及RGD-CS/pDNA复合体的形态，EB染色法检测钛片表面RGD-CS/pDNA复合体的接枝效果。结果 红外光谱检测结合元素分析表明，CS和RGD肽偶联成功；凝胶电泳阻滞试验结合原子力显微镜观察表明，在N/P≥2时，RGD-CS与pDNA完全复合，RGD-CS/pDNA复合体呈类球形；EB染色表明RGD-CS/pDNA复合体接枝钛片成功。结论 经RGD肽修饰的壳聚糖可以携带pDNA作为钛种植体表面质粒包装的载体。
Bibliography:51-1169/R
Zhang Di, Liu Changhong, Zhang Jincai, Cai Dehong, Yang Xiaoyu, Li Shiyi, Zhong Huilan（ 1. Dept. of Implantology, The Affiliated Stomatological Hospital of Southern Medical University and Guangdong Provincial Stomatological Hospital, Guangzhou 510280, China; 2. Dept. of Endocrinology, The Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510282, China ）
RGD peptide; titanium; chitosan; implant
Objective This study is conducted to explore new methods to perform surface biomodification of titanium implants and improve osteogenic efficiency. Methods An RGD peptide and chitosan （CS） were combined by acylation reaction, forming RGD-CS. An RGD-CS/pDNA complex was subsequently prepared using a complex coacervation method and grafted on a pure titanium surface after physical and biochemical treatments were performed. The chemical structural characteristics of RGD-CS were evaluated using an infrared spectrometer and an elemental analyzer. The shape of this complex was then assessed by gel e
ISSN:1000-1182
DOI:10.7518/hxkq.2014.04.005