藏药蕨麻多糖的光谱性质及单糖组成分析

本研究对藏药蕨麻多糖进行了分离提纯,并测定其水溶性多糖含量为99.4%;通过紫外光谱与红外光谱分析表明,蕨麻多糖为分子量较小的α-吡喃糖,并含有氨基糖;蕨麻多糖的水解单糖经过NMP衍生后进行毛细管电泳分析,测得其单糖组成为木糖、阿拉伯糖、葡萄糖、鼠李糖、甘露糖、岩藻糖、半乳糖、葡萄糖醛酸和半乳糖醛酸,含量分别为3.945、77.445、17.568、17.646、3.942、2.165、65.268、13.037μg/mg和33.484μg/mg,与GC-MS的定性分析结果一致。...

Full description

Saved in:
Bibliographic Details
Published in天然产物研究与开发 Vol. 23; no. 3; pp. 453 - 457
Main Author 夏莲 孙志伟 李国梁 索有瑞 尤进茂
Format Journal Article
LanguageChinese
Published 中国科学院西北高原生物研究所,西宁,810001 2011
中国科学院研究生院,北京,1000491%中国科学院西北高原生物研究所,西宁,810001%中国科学院西北高原生物研究所,西宁,810001
山东省曲阜师范大学化学与化工学院生命有机分析重点实验室,曲阜,273165
中国科学院研究生院,北京,1000491%中国科学院西北高原生物研究所,西宁,810001
Subjects
光谱性质
单糖组成
多糖
蕨麻
单糖组成
蕨麻
光谱性质
多糖
Online AccessGet full text
ISSN1001-6880
DOI10.3969/j.issn.1001-6880.2011.03.016

Cover

More Information
Summary:本研究对藏药蕨麻多糖进行了分离提纯,并测定其水溶性多糖含量为99.4%;通过紫外光谱与红外光谱分析表明,蕨麻多糖为分子量较小的α-吡喃糖,并含有氨基糖;蕨麻多糖的水解单糖经过NMP衍生后进行毛细管电泳分析,测得其单糖组成为木糖、阿拉伯糖、葡萄糖、鼠李糖、甘露糖、岩藻糖、半乳糖、葡萄糖醛酸和半乳糖醛酸,含量分别为3.945、77.445、17.568、17.646、3.942、2.165、65.268、13.037μg/mg和33.484μg/mg,与GC-MS的定性分析结果一致。
Bibliography:The polysaccharide of Potentilla anserine L.was extracted and purified,and its contents was determined as 98.4% by phenyl-sulfuric acid method.The polysaccharide was identified by IR spectrum and UV scanning spectrum.The IR spectrum indicates that the characteristic absorption peaks at 3600-3200,3200-2800,1400-1200,1200-1000 cm-1,and 845 cm-1 belonged the characteristic peak of α-pyranose.In addition,the peak at 1630 cm-1 assigned to the C=O of acetamide moiety stretching vibration meant that amino sugars exited in the polysaccharide.A method was developed for the separation of derivatized carbohydrates of Potentilla anserine L.using 1-naphthyl-3-methyl-5-pyrazolone（NMP） as derivatization reagent by capillary zone electrophoresis, and the results shows that the monosaccharides compositions of the polysaccharide from Potentilla anserine L.are xylose, arabinose,glucose,rhamnose,mannose,fucose,galactose,glucuronic acid and galacturonic acid with contents of 3.945,77.445, 17.568,17.646,3.942,2.165,65.268,13.037 μ
ISSN:1001-6880
DOI:10.3969/j.issn.1001-6880.2011.03.016