骨形态发生蛋白2对成牙骨质细胞中硬化蛋白表达调控机理的研究

目的探索成牙骨质细胞OCCM-30中骨形态发生蛋白2（BMP2）对硬化蛋白（SOST）表达的调控机制。方法用2种质量浓度的BMP2（50、100 ng·mL-1）处理成牙骨质OCCM-30细胞3、5、7 d,相同体积的PBS液为对照组,采用实时荧光定量聚合酶链反应（RT-PCR）、免疫印迹法检测SOST m RNA和蛋白的表达情况。将OCCM-30细胞分为5组：空白对照组、BMP2组、BMP2＋dorsomorphin组、BMP2＋SB202190组、BMP2＋PD98059组,根据分组分别加入100 ng·mL-1的BMP2和相应的试剂共培养,于3、5 d时检测SOST m RNA和蛋白的表...

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Bibliographic Details
Published in华西口腔医学杂志 Vol. 34; no. 3; pp. 244 - 247
Main Author 陈悦 李书琴 黄兰 戴红卫
Format Journal Article
LanguageChinese
Published 重庆医科大学附属口腔医院正畸科·口腔疾病与生物医学重庆市重点实验室·重庆市高校市级口腔生物医学工程重点实验室，重庆 401147 2016
Subjects
Smad信号通路
成牙骨质细胞
牙根修复
硬化蛋白
骨形态发生蛋白2
cementoblast
骨形态发生蛋白2
牙根修复
Smad信号通路
成牙骨质细胞
硬化蛋白
bone morphogenetic protein 2
Smad signal pathway
root repair
sclerostin
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ISSN1000-1182
DOI10.7518/hxkq.2016.03.006

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Summary:目的探索成牙骨质细胞OCCM-30中骨形态发生蛋白2（BMP2）对硬化蛋白（SOST）表达的调控机制。方法用2种质量浓度的BMP2（50、100 ng·mL-1）处理成牙骨质OCCM-30细胞3、5、7 d,相同体积的PBS液为对照组,采用实时荧光定量聚合酶链反应（RT-PCR）、免疫印迹法检测SOST m RNA和蛋白的表达情况。将OCCM-30细胞分为5组：空白对照组、BMP2组、BMP2＋dorsomorphin组、BMP2＋SB202190组、BMP2＋PD98059组,根据分组分别加入100 ng·mL-1的BMP2和相应的试剂共培养,于3、5 d时检测SOST m RNA和蛋白的表达情况。结果 100 ng·mL-1 BMP2对SOST表达的上调作用强于50 ng·mL-1 BMP2,且有时间依赖性（P〈0.05）。BMP2＋dorsomorphin组、BMP2＋SB202190组、BMP2＋PD98059组的SOST m RNA水平和蛋白质水平均降低,其中BMP2＋dorsomorphin组降低最明显（P〈0.05）。结论成牙骨质细胞中BMP2主要是通过Smad信号通路介导上调SOST的表达。
Bibliography:51-1169/R
cementoblast; bone morphogenetic protein 2; sclerostin; Smad signal pathway; root repair
Chen Yue,Li Shuqin,Huang Lan,Dai Hongwei（Dept.of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China）
Objective This research explores the regulatory role of bone morphogenetic protein 2（BMP2） in the expression of sclerostin in OCCM-30 cementoblast. Methods OCCM-30 cementoblasts were treated with 50 and 100 ng·mL-1 BMP2 for 3, 5, and 7 days. SOST m RNA was detected by real-time quantitative polymerase chain reaction（RT-PCR）. Western blot analysis was employed to detect the sclerostin levels in the nucleus. Five groups were prepared for the experiments： control, BMP2, BMP2＋dorsomorphin, BMP2＋SB202190, and BMP2＋PD98059. OCCM-30 was pretreated with BMP2 for 3 and 5 days, and then the sclerostin and SOST m RNA l
ISSN:1000-1182
DOI:10.7518/hxkq.2016.03.006