TYLCV山东分离物株系分化及侵染性克隆的构建

对2012~2014年山东省96份感番茄黄化曲叶病毒（TYLCV）样品进行特异性检测,发现有94份样品感染TYLCV,有2份样品感染番茄卷叶病毒（To LCV）。对该94份样品进行全序列克隆和序列测定,共分离出13份TYLCV株系,经核苷酸序列相似性分析和系统进化树构建,明确侵染我国山东地区的TYLCV为TYLCV-IL株系,且3年间爆发于山东省内的TYLCV株系的DNA组分并未发生大变异。遂构建山东寿光TYLCV株系侵染性克隆,经致病性验证,具有较好的侵染率,为番茄抗Ty育种提供稳定的毒源筛选压力。...

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Bibliographic Details
Published in东北农业大学学报 Vol. 46; no. 1; pp. 26 - 33
Main Author 薛东齐 刘冠 姜景彬 许向阳 李景富
Format Journal Article
LanguageChinese
Published 东北农业大学园艺学院,哈尔滨,150030 2015
Subjects
侵染性克隆
特异性检测
番茄黄化曲叶病毒
系统进化树
番茄黄化曲叶病毒
phylogenetic tree
系统进化树
tomato yel ow leaf curl virus
infectious clone
specific detection
侵染性克隆
特异性检测
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ISSN1005-9369

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Summary:对2012~2014年山东省96份感番茄黄化曲叶病毒（TYLCV）样品进行特异性检测,发现有94份样品感染TYLCV,有2份样品感染番茄卷叶病毒（To LCV）。对该94份样品进行全序列克隆和序列测定,共分离出13份TYLCV株系,经核苷酸序列相似性分析和系统进化树构建,明确侵染我国山东地区的TYLCV为TYLCV-IL株系,且3年间爆发于山东省内的TYLCV株系的DNA组分并未发生大变异。遂构建山东寿光TYLCV株系侵染性克隆,经致病性验证,具有较好的侵染率,为番茄抗Ty育种提供稳定的毒源筛选压力。
Bibliography:XUE Dongqi;LIU Guan;JIANG Jingbin;XU Xiangyang;LI Jingfu （School of Horticulture, Northeast Agricultural University, Harbin 150030, China）
23-1391/S
tomato yellow leaf curl virus; specific detection; phylogenetic tree; infectious clone
There were 96 samples of a sense of tomato yellow leaf curl virus（TYLCV） that were collected in Shandong Province between 2012- 2014. After specific detection, 94 samples infected TYLCV and two samples infected To LCV. The 94 samples that infected TYLCV were cloned and sequenced the whole sequence. The results showed that 13 TYLCV strains were separated. By nucleotide sequence similarity analysis and phylogenetic tree construction, We identified that the TYLCV isolates infected Shandong area belonged TYLCV-IL strains, and TYLCV DNA component of Shandong Province didn＇t occur large variation between 2012- 2014. Then we constructed Shandong Shouguang TYLCV strain infectious clone, By pathogenic verification, It had a better infection rate. Therefore, It provide a stable viral source
ISSN:1005-9369