Nonsaponin fractions of Korean Red Ginseng extracts prime activation of NLRP3 inflammasome
Korean Red Ginseng extracts (RGE) have been suggested as effective immune modulators, and we reported that ginsenosides possess anti-inflammasome properties. However, the properties of nonsaponin components of RGE have not been well studied. To assess the roles of nonsaponin fractions (NS) in NLRP3...
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Published in | Journal of ginseng research Vol. 41; no. 4; pp. 513 - 523 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Korea (South)
Elsevier B.V
01.10.2017
고려인삼학회 Elsevier |
Subjects | |
Online Access | Get full text |
ISSN | 1226-8453 2093-4947 2093-4947 |
DOI | 10.1016/j.jgr.2016.10.001 |
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Summary: | Korean Red Ginseng extracts (RGE) have been suggested as effective immune modulators, and we reported that ginsenosides possess anti-inflammasome properties. However, the properties of nonsaponin components of RGE have not been well studied.
To assess the roles of nonsaponin fractions (NS) in NLRP3 inflammasome activation, we treated murine macrophages with or without first or second inflammasome activation signals with RGE, NS, or saponin fractions (SF). The first signal was nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB)-mediated transcription of pro-interleukin (IL)-1β and NLRP3 while the second signal triggered assembly of inflammasome components, leading to IL-1β maturation. In addition, we examined the role of NS in IL-6 production and IL-1β maturation in mice.
NS induced IL-1β and NLRP3 transcription via toll-like receptor 4 signaling, whereas SF blocked expression. During the second signal, SF attenuated NLRP3 inflammasome activation while NS did not. Further, NS-injected mice presented increased IL-1β maturation and IL-6 production.
SF and NS of RGE play differential roles in the NLRP3 inflammasome activation. Hence, RGE can be suggested as an NLRP3 inflammasome modulator. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work. |
ISSN: | 1226-8453 2093-4947 2093-4947 |
DOI: | 10.1016/j.jgr.2016.10.001 |