P120-连环蛋白介导钙黏蛋白转换促进口腔鳞状细胞癌细胞迁移和侵袭的实验研究

目的 探索P120-连环蛋白（P120^ctn）在口腔鳞状细胞癌（OSCC）细胞钙黏蛋白转换中的作用及其对肿瘤细胞迁移和侵袭的影响。方法 采用质粒p GFP-V-RS-P120^ctn sh RNA转染OSCC细胞株TSCCA,采用实时荧光定量聚合酶链反应、Western blot检测细胞中P120^ctn、E-钙黏蛋白（E-cad）和N-钙黏蛋白（N-cad）的m RNA和蛋白表达的变化,通过Transwell细胞侵袭及细胞迁移实验检测转染前后细胞迁移和侵袭能力的变化。结果 质粒p GFP-V-RSP120^ctn sh RNA转染TSCCA细胞后,P120^ctn表达明显降低,E-cad的...

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Bibliographic Details
Published in华西口腔医学杂志 Vol. 35; no. 2; pp. 183 - 186
Main Author 陈钟 张美 徐勇
Format Journal Article
LanguageChinese
Published 厦门医学院口腔医学系,厦门,361008%山东省高青县人民医院口腔科,高青,256300 2017
Subjects
P120-连环蛋白
口腔鳞状细胞癌
钙黏蛋白转换
钙黏蛋白转换
P120-catenin
口腔鳞状细胞癌
oral squamous cell cancer
P120-连环蛋白
cadherin switching
Online AccessGet full text
ISSN1000-1182
DOI10.7518/hxkq.2017.02.014

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Summary:目的 探索P120-连环蛋白（P120^ctn）在口腔鳞状细胞癌（OSCC）细胞钙黏蛋白转换中的作用及其对肿瘤细胞迁移和侵袭的影响。方法 采用质粒p GFP-V-RS-P120^ctn sh RNA转染OSCC细胞株TSCCA,采用实时荧光定量聚合酶链反应、Western blot检测细胞中P120^ctn、E-钙黏蛋白（E-cad）和N-钙黏蛋白（N-cad）的m RNA和蛋白表达的变化,通过Transwell细胞侵袭及细胞迁移实验检测转染前后细胞迁移和侵袭能力的变化。结果 质粒p GFP-V-RSP120^ctn sh RNA转染TSCCA细胞后,P120^ctn表达明显降低,E-cad的m RNA和蛋白表达水平也明显降低,而N-cad的m RNA和蛋白表达水平明显提高,细胞迁移和侵袭能力也明显提高,差异均有统计学意义（P〈0.05）。结论 在OSCC中P120^ctn可能通过介导钙黏蛋白转换来调节肿瘤的浸润和转移过程。
Bibliography:51-1169/R
P120-catenin; cadherin switching; oral squamous cell cancer
Objective The main goal is to investigate the role of P120-catenin （P120^ctn） in cadherin switching, as well as migration and invasion, of oral squamous cell cancer （OSCC） cells. Methods The plasmid pGFP-V-RS-P120^ctn shRNA was used to transfect TSCCA cells and significantly reduce the expression of P120^ctn in these cells. Real-time fluorescent quantita-tive polymerase chain reaction and Western blot were conducted to determine the mRNA and protein expression levels of P120^ctn, E-cadherin （E-cad）, and N-cadherin （N-cad）. By contrast, the Transwell cell invasion and cell migration assay was used to determine the invasion and migration capacities before and after the transfection. Results After the plasmid pGFP-V-RS-P120^ctn shRNA was transfected into the TSCCA cells, we found that as the P120^ctn expression significantly decreased, E-cad mRNA and protein expression decreased significantly. Moreover, N-cad mRNA and protein expression increase
ISSN:1000-1182
DOI:10.7518/hxkq.2017.02.014