C/EBPβ在人正常肝细胞和肝癌细胞系中的差异表达及其与内质网应激相关细胞死亡的关系

目的 观察转录因子C/EBPβ在人永生化正常肝细胞和肝癌细胞系中的表达差异性,探讨其与肝癌细胞内质网应激介导的细胞死亡的相关性。方法 培养人永生化正常肝细胞系HHL5、HL7702和肝癌SMMC7721、Bel7402、HepG2、Hep3B细胞系;Hep3B细胞用衣霉素诱导内质网应激细胞模型;倒置光学显微镜下观察细胞形态;MTT法检测细胞生长抑制率;Hoechst 33258染色后荧光显微镜下检测细胞凋亡;RT-PCR和Western blotting技术分别检测mRNA和蛋白的表达水平。结果 C/EBPβ的mRNA和蛋白亚型C/EBPβ-1在4种人肝癌细胞系和永生化正常肝细胞系中均有表达,...

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Bibliographic Details
Published in西安交通大学学报（医学版） Vol. 38; no. 4; pp. 487 - 491
Main Author 卢新兰 卢桂芳 王新 张俊 张丹 赵艳 闫红林 任牡丹 和水祥
Format Journal Article
LanguageChinese
Published 西安交通大学第一附属医院消化内科,陕西西安,710061%西安医学院第一附属医院消化内科,陕西西安,710077 2017
Subjects
C/EBPβ
内质网应激
类凋亡
细胞凋亡
肝细胞癌
C/EBPβ
细胞凋亡
肝细胞癌
paraptosis
apoptosis
内质网应激
类凋亡
hepatocellular carcinoma
endoplasmic reticulum stress
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ISSN1671-8259
DOI10.7652/jdyxb201704004

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Summary:目的 观察转录因子C/EBPβ在人永生化正常肝细胞和肝癌细胞系中的表达差异性,探讨其与肝癌细胞内质网应激介导的细胞死亡的相关性。方法 培养人永生化正常肝细胞系HHL5、HL7702和肝癌SMMC7721、Bel7402、HepG2、Hep3B细胞系;Hep3B细胞用衣霉素诱导内质网应激细胞模型;倒置光学显微镜下观察细胞形态;MTT法检测细胞生长抑制率;Hoechst 33258染色后荧光显微镜下检测细胞凋亡;RT-PCR和Western blotting技术分别检测mRNA和蛋白的表达水平。结果 C/EBPβ的mRNA和蛋白亚型C/EBPβ-1在4种人肝癌细胞系和永生化正常肝细胞系中均有表达,但C/EBPβ-3仅在人永生化正常肝细胞系中表达,在肝癌细胞系中不表达;衣霉素能够上调人肝癌Hep3B细胞C/EBPβ的mRNA和蛋白表达水平,明显上调C/EBPβ-3的表达水平;衣霉素能够诱导人肝癌Hep3B细胞内质网应激介导的细胞死亡,表现为细胞凋亡和类凋亡两种方式。结论 C/EBPβ的蛋白亚型C/EBPβ-3在人永生化正常肝细胞中表达而在肝癌细胞中不表达;C/EBPβ参与内质网应激介导的人肝癌细胞死亡。
Bibliography:hepatocellular carcinoma; C/EBPβ endoplasmic reticulum stress; apoptosis; paraptosis
LU Xin-lan1 , LU Gui-fang1 WANG Kin1, ZHANG Jun1 , ZHANG Dan1 , ZHAO Yan1 , YAN Hong-lin2 , REN Mu-dan1 , HE Shui-xiang1 （1. Department of Gastroenterology, the First Affiliated Hospital of an Jiaotong University, Xi＇an 710061; 2. Department of Gastroenterology, the First Affiliated Hospital of Medical College, Xi＇an 710077, China）
Objective To detect the expression profile of transcription factor C/EBPβ in human immortalized normal hepatic cell lines and hepatocellular carcinoma cell lines so as to determine the correlation between C/EBPβ with cell death mediated by endoplasmic reticulum stress in hepatocellular cells. Methods We cultured the human immortalized normal hepatic cells lines HHL5 and HL7702 and hepatocellular carcinoma cell lines SMMC7721; Be17402, HepG2 and Hep3B. Hep3B cells were used as the cell model in tunicamycin-induced endoplasmic reticulum stress. Cellular morphology was observed under an inverted optical
ISSN:1671-8259
DOI:10.7652/jdyxb201704004