蜜蜂巢脾抗氧化活性与抗菌活性的研究

以意蜂巢脾和中蜂巢脾为研究材料,分别采用水提法和醇提法处理,对获得的4种提取物,以DPPH.法测定自由基清除能力,福林酚法测定总酚含量,琼脂扩散法测定其对金黄色葡萄球菌和大肠杆菌的抑菌半径。结果表明,巢脾提取物具有一定的抗氧化活性和抑菌活性,且含有丰富的酚类化合物。除了对大肠杆菌的抑制作用外,巢脾水提液效果优于巢脾醇提液;除DPPH.清除能力外,意蜂巢脾效果优于中蜂巢脾效果;生物学活性呈现浓度依赖效应。本研究为巢脾在中医药领域的应用提供了一定的解释,并证明了巢脾是一种潜在的天然生物资源。...

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Bibliographic Details
Published in天然产物研究与开发 Vol. 23; no. 4; pp. 726 - 729
Main Author 褚亚芳 胡福良
Format Journal Article
LanguageChinese
Published 浙江大学动物科学学院,杭州,310029 2011
Subjects
DPPH
中蜂巢脾
总酚含量
意蜂巢脾
抑菌活性
中蜂巢脾
总酚含量
意蜂巢脾
DPPH
抑菌活性
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ISSN1001-6880
DOI10.3969/j.issn.1001-6880.2011.04.032

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Summary:以意蜂巢脾和中蜂巢脾为研究材料,分别采用水提法和醇提法处理,对获得的4种提取物,以DPPH.法测定自由基清除能力,福林酚法测定总酚含量,琼脂扩散法测定其对金黄色葡萄球菌和大肠杆菌的抑菌半径。结果表明,巢脾提取物具有一定的抗氧化活性和抑菌活性,且含有丰富的酚类化合物。除了对大肠杆菌的抑制作用外,巢脾水提液效果优于巢脾醇提液;除DPPH.清除能力外,意蜂巢脾效果优于中蜂巢脾效果;生物学活性呈现浓度依赖效应。本研究为巢脾在中医药领域的应用提供了一定的解释,并证明了巢脾是一种潜在的天然生物资源。
Bibliography:Apis cerana cerana honeycomb; A.mellifera ligustica honeycomb; DPPH·; total phenolic contents; antimicrobial ability
Water extracts and ethanol extracts were prepared from Apis cerana cerana honeycomb and A.mellifera ligustica honeycomb,both of which were used more than one year.The antioxidant activities of the 4 different type extracts were evaluated using the 2,2-diphenyl-1-picrylhydrazyl radical（DPPH·） assay,while the total phenolic contents of these samples were measured by the Folin-Ciocalteu reagent method,respectively.Furthermore,the antimicrobial capabilities of the extracts were measured by the paper disc-agar diffusion method against Staphylococcus aureus and Escherichia coli.The results showed that all the 4 types of extracts of honeycomb were capable of scavenging free radicals and inhibiting both Staphylococcus aureus and Escherichia coli to some extent and rich in phenolic.Totally speaking,the water extracts perform better than the ethanol extracts in all except the antimicrobial ability againt
ISSN:1001-6880
DOI:10.3969/j.issn.1001-6880.2011.04.032