上海地区汉族人群缺血性卒中与NADPH氧化酶p22phox亚基基因多态性相关性研究

目的研究上海地区汉族人群动脉粥样硬化性缺血性卒中患者烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶p22phox亚基-930A/G、242C/T、-675A/T基因型和等位基因频率,探讨单核苷酸多态性与缺血性卒中的关联性。方法采用聚合酶链反应-限制性片段长度多态性技术测定205例缺血性卒中患者和136例正常对照者NADPH氧化酶p22phox亚基-930A/G、242C/T和-675A/T基因型和等位基因频率。结果缺血性卒中患者NADPH氧化酶p22phox亚基-930A/G位点AA型于268 bp处呈现1条电泳条带,GG型于197和71 bp处呈现2条条带,AG型于268、197和71 bp处...

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Bibliographic Details
Published in中国现代神经疾病杂志 Vol. 15; no. 9; pp. 739 - 745
Main Author 徐玮 傅毅 何明 倪培华 宋永建
Format Journal Article
LanguageChinese
Published 200025,上海交通大学医学院附属瑞金医院神经科%200020,上海交通大学医学院附属瑞金医院卢湾分院神经内科%200025,上海交通大学医学院附属瑞金医院检验系 2015
Subjects
NADPH氧化酶
上海
多态现象
汉族
脑缺血
遗传
多态现象,遗传
Polymorphism,genetic
脑缺血
上海
Shanghai
NADPH oxidase
Brain ischemia
Han nationality
汉族
NADPH氧化酶
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ISSN1672-6731
DOI10.3969/j.issn.1672-6731.2015.09.011

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Summary:目的研究上海地区汉族人群动脉粥样硬化性缺血性卒中患者烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶p22phox亚基-930A/G、242C/T、-675A/T基因型和等位基因频率,探讨单核苷酸多态性与缺血性卒中的关联性。方法采用聚合酶链反应-限制性片段长度多态性技术测定205例缺血性卒中患者和136例正常对照者NADPH氧化酶p22phox亚基-930A/G、242C/T和-675A/T基因型和等位基因频率。结果缺血性卒中患者NADPH氧化酶p22phox亚基-930A/G位点AA型于268 bp处呈现1条电泳条带,GG型于197和71 bp处呈现2条条带,AG型于268、197和71 bp处呈现3条条带;242C/T位点CC型于348 bp处呈现1条电泳条带,TT型于188和160 bp处呈现2条条带,CT型于348、188和160 bp处呈现3条条带;-675A/T位点TT型于158和54 bp处呈现2条电泳条带,AT型于212、158和54 bp处呈现3条条带。对照组与脑缺血组受试者基因型和等位基因频率比较,差异均无统计学意义（P〉0.05）。结论NADPH氧化酶p22phox亚基-930A/G、242C/T、-675A/T位点基因多态性与缺血性卒中的发病可能并无直接关联性。
Bibliography:Objective This paper aims to investigate the distribution of genotypes and alleles of nieotinamide adenine dinucleotide phosphate （NADPH） oxidase p22phox - 930A/G, 242C/T and - 675A/T, so as to evaluate the association between three single- nueleotide polymorphisms （SNPs） and risk of atheroselerotic isehemie stroke in permanent resident population of Han nationality living in Shanghai area. Methods The genotypes and allele frequencies of NADPH oxidase p22phox subunit - 930A/G, 242C/T and -675A/T were detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） analysis in 205 patients with ischemic stroke and 136 healthy controls. Results In patients with ischemic stroke, the results of PCR-RFLP in variant genetic loci were different. For - 930A/G, one band appeared at 268 bp of genotype AA; 2 bands appeared at 197 and 71 bp of genotype GG; 3 bands appeared at 268, 197 and 71 bp of genotype AG. For 242C/T, one band appeared at 348 bp of genotype CC; 2 bands appeared at 188 and 160
ISSN:1672-6731
DOI:10.3969/j.issn.1672-6731.2015.09.011