Development of microsatellite markers for the Japanese endemic conifer Thuja standishii and transfer to other East Asian species

• Published in: BMC research notes Vol. 12; no. 1; pp. 694 - 5
• Main Authors: Worth, James R. P., Chang, K. S., Ha, Y.-H., Qin, Aili
• Format: Journal Article
• Language: English
• Published: London BioMed Central 25.10.2019; BioMed Central Ltd; BMC
• Subjects: Alleles; Anopheles; Arbor vitae; Asian Continental Ancestry Group; Biodiversity; Biomedical and Life Sciences; Biomedicine; China; Conifer; DNA, Plant - genetics; Gene Flow; Genes; Genetic aspects; Genetic Loci; Genetic research; Genotype; Humans; Japan; Japanese arbor-vitae; Korea; Life Sciences; Medicine/Public Health; Microsatellite Repeats - genetics; Microsatellites (Genetics); Nuclear microsatellites; Polymorphism, Genetic; Research Note; Species Specificity; Thuja; Thuja - classification; Thuja - genetics; Thuja - growth & development; Tracheophyta - classification; Tracheophyta - genetics; Tracheophyta - growth & development; Varieties; Japan; Japanese arbor-vitae; China; Conifer; Nuclear microsatellites; Korea; Thuja
• ISSN: 1756-0500; 1756-0500
• DOI: 10.1186/s13104-019-4716-z

Objective Design polymorphic microsatellite loci that will be useful for studies of the genetic diversity, gene-flow and reproduction in the Japanese endemic conifer Thuja standishii and test the transferability of these loci to the two other East Asian species, T. sutchuenensis and T. koraiensis . Results Fifteen loci were developed which displayed 3 to 21 alleles per locus (average = 9.2) among 97 samples from three populations of T. standishii . Observed heterozygosity for all samples varied between 0.33 and 0.75 (average = 0.54) while expected heterozygosity values were higher with an average over the 15 loci of 0.62 (0.37–0.91). Low multi-locus probability of identity values (< 0.00002) indicate that these markers will be effective for identifying individuals derived from clonal reproduction. All 15 loci amplified in 13 samples of T. sutchuenensis , the sister species of T. standishii , with 1 to 11 alleles per locus (average = 4.33) while 13 loci amplified in four samples of the more distantly related T. koraiensis with 1 to 5 alleles per locus (average = 2.15).