Prevalence of CTX-M types among ESBL-producing pathogenic Escherichia coli isolates from foodborne diarrheal patients in Gyeonggi-do, South Korea

• Published in: Food science and biotechnology Vol. 33; no. 12; pp. 2825 - 2833
• Main Authors: Park, Nanjoo, Hur, Jeong In, Lee, Sohyun, Ryu, Sangryeol
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01.09.2024; Springer Nature B.V; 한국식품과학회
• Subjects: Antibiotic resistance; beta-lactamase bacteria; Chemistry; Chemistry and Materials Science; computer simulation; Diarrhea; Disease resistance; E coli; enterotoxigenic Escherichia coli; Escherichia coli; Food Science; Infectious diseases; M gene; Nucleotides; Nutrition; pathotypes; Phylogeny; Public health; Quinolones; Research Article; resistance genes; risk; South Korea; β Lactamase; 식품과학; South Korea; Foodborne outbreaks; Extended-spectrum β-lactamase (ESBL); Plasmid-mediated quinolone resistance genes (PMQR); Pathogenic; Antibiotic resistance; Pathogenic Escherichia coli
• ISSN: 1226-7708; 2092-6456; 2092-6456
• DOI: 10.1007/s10068-024-01549-5

Prevalence and characteristics of extended-spectrum β-lactamase (ESBL)-producing pathogenic Escherichia coli from foodborne diarrheal patients were studied. Analysis of 495 E. coli isolates revealed that 80 isolates were ESBL-producing pathogenic E. coli , and enteroaggregative E. coli and enterotoxigenic E. coli were two of the most prevalent pathotypes. In silico Clermont phylo-typing of the 80 ESBL-producing E. coli showed that phylogroup A (49/80) and D (22/80) were the predominant phylogroups. The average nucleotide identity analysis of ESBL-producing E. coli disclosed that they could be grouped into two phylogenetic groups; 25 A and 55 B groups. All strains, except one, harbored the bla CTX-M gene. All CTX-M-15 type ESBL-producing strains also carried qnrS , a plasmid-mediated quinolone resistance gene (PMQR). These results suggest that the diversity of ESBL-producing E. coli is high and that co-existence of bla CTX-M-15 and qnrS genes is widespread, highlighting their high risk of antibiotic-resistance spreading in infectious disease outbreaks.