拟南芥中RING型E3泛素连接酶基因AtGW2的克隆和功能分析
水稻R ING型E3泛素连接酶基因O sGW2在调控水稻产量性状方面起着十分重要的作用。根据O sGW2的cDNA序列,通过RT-PCR方法从拟南芥中克隆了一个与O sGW2同源的基因,命名为AtGW2。序列分析表明,该基因编码一个R ING-C2型E3泛素连接酶蛋白,含有401个氨基酸。通过构建AtGW2RNA干扰植物表达载体并转化拟南芥,结果表明,获得的转基因后代植株的子粒较野生型大,并且转基因拟南芥子粒千粒重高于野生型,这表明AtGW2负调控拟南芥子粒大小及粒重。...
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| Published in | 植物遗传资源学报 Vol. 12; no. 3; pp. 448 - 454 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
黑龙江大学农作物研究院,哈尔滨,150080
2011
河南省农业科学院粮食作物研究所/河南省玉米生物学重点实验室,郑州,450002%河南省农业科学院粮食作物研究所/河南省玉米生物学重点实验室,郑州,450002%黑龙江大学农作物研究院,哈尔滨,150080 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1672-1810 |
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| Abstract | 水稻R ING型E3泛素连接酶基因O sGW2在调控水稻产量性状方面起着十分重要的作用。根据O sGW2的cDNA序列,通过RT-PCR方法从拟南芥中克隆了一个与O sGW2同源的基因,命名为AtGW2。序列分析表明,该基因编码一个R ING-C2型E3泛素连接酶蛋白,含有401个氨基酸。通过构建AtGW2RNA干扰植物表达载体并转化拟南芥,结果表明,获得的转基因后代植株的子粒较野生型大,并且转基因拟南芥子粒千粒重高于野生型,这表明AtGW2负调控拟南芥子粒大小及粒重。 |
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| AbstractList | Q943.2; 水稻RING型E3泛素连接酶基因OsGW2在调控水稻产量性状方面起着十分重要的作用.根据OsGW2的cDNA序列,通过RT-PCR方法从拟南芥中克隆了一个与OsGW2同源的基因,命名为AtGW2.序列分析表明,该基因编码一个RING-C2型E3泛素连接酶蛋白,含有401个氨基酸.通过构建AtGW2 RNA干扰植物表达栽体并转化拟南芥,结果表明,获得的转基因后代植株的子粒较野生型大,并且转基因拟南芥子粒千粒重高于野生型,这表明AtGW2负调控拟南芥子粒大小及粒重. 水稻R ING型E3泛素连接酶基因O sGW2在调控水稻产量性状方面起着十分重要的作用。根据O sGW2的cDNA序列,通过RT-PCR方法从拟南芥中克隆了一个与O sGW2同源的基因,命名为AtGW2。序列分析表明,该基因编码一个R ING-C2型E3泛素连接酶蛋白,含有401个氨基酸。通过构建AtGW2RNA干扰植物表达载体并转化拟南芥,结果表明,获得的转基因后代植株的子粒较野生型大,并且转基因拟南芥子粒千粒重高于野生型,这表明AtGW2负调控拟南芥子粒大小及粒重。 |
| Author | 姜颖 曹言勇 路运才 唐保军 王利锋 李会勇 |
| AuthorAffiliation | 黑龙江大学农作物研究院,哈尔滨150080 河南省农业科学院粮食作物研究所／河南省玉米生物学重点实验室,郑州450002 |
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| Author_FL | LU Yun-cai CAO Yan-yong LI Hui-yong WANG Li-feng JIANG Ying TANG Bao-jun |
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| DocumentTitleAlternate | Cloning and Functional Analysis of Arabidopsis thaliana AtGW2,a RING-type E3 Ubiquitin Ligase Protein |
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| Keywords | 拟南芥 RING型E3泛素连接酶 RNAi 粒重 AtGW2 |
| Language | Chinese |
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| Notes | Arabidopsis thaliana; AtGW2; RING-type E3 ubiquitin ligase; RNAi; Grain weight OsGW2（RING type E3 ubiquitin ligase）plays important roles in regulating rice yielding traits.According to the OsGW2 cDNA sequences,the homolog of OsGW2 was isolated from Arabidopsis thaliana by RT-PCR and designated as AtGW2.The predicted AtGW2 protein has 401 amino acids,and has a RING-type domain.The grain size and kilo kernels weight of wild-type Arabidopsis plants were lower than those of AtGW2 RNA interference transgenic plants.These results suggested that AtGW2 negatively regulates the grain size and weight in Arabidopsis. JIANG Ying,CAO Yan-yong,LU Yun-cai,TANG Bao-jun,WANG Li-feng,LI Hui-yong（1Institute for Crop Research,Heilongjiang University,Harbin 150080;2 Henan Provincial Key Laboratory for Maize Biology/Cereal Crop Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002） 11-4996/S |
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| PublicationTitle | 植物遗传资源学报 |
| PublicationTitleAlternate | Journal of Plant Genetic Resources |
| PublicationTitle_FL | JOURNAL OF PLANT GENETIC RESOURCES |
| PublicationYear | 2011 |
| Publisher | 黑龙江大学农作物研究院,哈尔滨,150080 河南省农业科学院粮食作物研究所/河南省玉米生物学重点实验室,郑州,450002%河南省农业科学院粮食作物研究所/河南省玉米生物学重点实验室,郑州,450002%黑龙江大学农作物研究院,哈尔滨,150080 |
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| Snippet | 水稻R ING型E3泛素连接酶基因O sGW2在调控水稻产量性状方面起着十分重要的作用。根据O sGW2的cDNA序列,通过RT-PCR方法从拟南芥中克隆了一个与O sGW2同源的基因,命名... Q943.2; 水稻RING型E3泛素连接酶基因OsGW2在调控水稻产量性状方面起着十分重要的作用.根据OsGW2的cDNA序列,通过RT-PCR方法从拟南芥中克隆了一个与OsGW2同源的基因,命名... |
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| SubjectTerms | AtGW2 RING型E3泛素连接酶 RNAi 拟南芥 粒重 |
| Title | 拟南芥中RING型E3泛素连接酶基因AtGW2的克隆和功能分析 |
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