拟南芥中RING型E3泛素连接酶基因AtGW2的克隆和功能分析

水稻R ING型E3泛素连接酶基因O sGW2在调控水稻产量性状方面起着十分重要的作用。根据O sGW2的cDNA序列,通过RT-PCR方法从拟南芥中克隆了一个与O sGW2同源的基因,命名为AtGW2。序列分析表明,该基因编码一个R ING-C2型E3泛素连接酶蛋白,含有401个氨基酸。通过构建AtGW2RNA干扰植物表达载体并转化拟南芥,结果表明,获得的转基因后代植株的子粒较野生型大,并且转基因拟南芥子粒千粒重高于野生型,这表明AtGW2负调控拟南芥子粒大小及粒重。...

Full description

Saved in:
Bibliographic Details
Published in植物遗传资源学报 Vol. 12; no. 3; pp. 448 - 454
Main Author 姜颖 曹言勇 路运才 唐保军 王利锋 李会勇
Format Journal Article
LanguageChinese
Published 黑龙江大学农作物研究院,哈尔滨,150080 2011
河南省农业科学院粮食作物研究所/河南省玉米生物学重点实验室,郑州,450002%河南省农业科学院粮食作物研究所/河南省玉米生物学重点实验室,郑州,450002%黑龙江大学农作物研究院,哈尔滨,150080
Subjects
AtGW2
RING型E3泛素连接酶
RNAi
拟南芥
粒重
拟南芥
RING型E3泛素连接酶
RNAi
粒重
AtGW2
Online AccessGet full text
ISSN1672-1810

Cover

More Information
Summary:水稻R ING型E3泛素连接酶基因O sGW2在调控水稻产量性状方面起着十分重要的作用。根据O sGW2的cDNA序列,通过RT-PCR方法从拟南芥中克隆了一个与O sGW2同源的基因,命名为AtGW2。序列分析表明,该基因编码一个R ING-C2型E3泛素连接酶蛋白,含有401个氨基酸。通过构建AtGW2RNA干扰植物表达载体并转化拟南芥,结果表明,获得的转基因后代植株的子粒较野生型大,并且转基因拟南芥子粒千粒重高于野生型,这表明AtGW2负调控拟南芥子粒大小及粒重。
Bibliography:Arabidopsis thaliana; AtGW2; RING-type E3 ubiquitin ligase; RNAi; Grain weight
OsGW2（RING type E3 ubiquitin ligase）plays important roles in regulating rice yielding traits.According to the OsGW2 cDNA sequences,the homolog of OsGW2 was isolated from Arabidopsis thaliana by RT-PCR and designated as AtGW2.The predicted AtGW2 protein has 401 amino acids,and has a RING-type domain.The grain size and kilo kernels weight of wild-type Arabidopsis plants were lower than those of AtGW2 RNA interference transgenic plants.These results suggested that AtGW2 negatively regulates the grain size and weight in Arabidopsis.
JIANG Ying,CAO Yan-yong,LU Yun-cai,TANG Bao-jun,WANG Li-feng,LI Hui-yong（1Institute for Crop Research,Heilongjiang University,Harbin 150080;2 Henan Provincial Key Laboratory for Maize Biology/Cereal Crop Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002）
11-4996/S
ISSN:1672-1810