Anti-angiogenic effect of siphonaxanthin from green alga, Codium fragile

• Published in: Phytomedicine (Stuttgart) Vol. 17; no. 14; pp. 1140 - 1144
• Main Authors: Ganesan, Ponesakki, Matsubara, Kiminori, Ohkubo, Takeshi, Tanaka, Yukihisa, Noda, Kenji, Sugawara, Tatsuya, Hirata, Takashi
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.12.2010; Urban & Fischer Verlag
• Subjects: Angiogenesis; Angiogenesis Inhibitors - isolation & purification; Angiogenesis Inhibitors - pharmacology; Animals; Antimitotic agents; Antineoplastic agents; Aorta; Aortic ring; Cardiovascular agents; Cell Proliferation - drug effects; Chlorophyta - chemistry; Codium fragile; Dose-Response Relationship, Drug; Drug therapy; Endothelial cells; Endothelial Cells - drug effects; Endothelium, Vascular - cytology; Endothelium, Vascular - drug effects; Health aspects; Humans; Medical equipment and supplies industry; Medical test kit industry; Metastasis; Microvessels - drug effects; Plant Extracts - pharmacology; Rats; Siphonaxanthin; Umbilical Veins; Vascular endothelial growth factor; Xanthophylls - isolation & purification; Xanthophylls - pharmacology; United States; Japan; Angiogenesis; Siphonaxanthin; Endothelial cells; Aortic ring
• ISSN: 0944-7113; 1618-095X; 1618-095X
• DOI: 10.1016/j.phymed.2010.05.005

Since anti-angiogenic therapy has becoming a promising approach in the prevention of cancer and related diseases, the present study was aimed to examine the anti-angiogenic effect of siphonaxanthin from green alga ( Codium fragile) in cell culture model systems and ex vivo approaches using human umbilical vein endothelial cells (HUVECs) and rat aortic ring, respectively. Siphonaxanthin significantly suppressed HUVEC proliferation ( p < 0.05) at the concentration of 2.5 μM (50% as compared with control) and above, while the effect on chemotaxis was not significant. Siphonaxanthin exhibited strong inhibitory effect on HUVEC tube formation. It suppressed the formation of tube length by 44% at the concentration of 10 μM, while no tube formation was observed at 25 μM, suggesting that it could be due to the suppression of angiogenic mediators. The ex vivo angiogenesis assay exhibited reduced microvessel outgrowth in a dose dependent manner and the reduction was significant at more than 2.5 μM. Our results imply a new insight on the novel function of siphonaxanthin in preventing angiogenesis related diseases.