甘蔗野生种滇蔗茅种质创新利用研究I．甘蔗与滇蔗茅远缘杂交F1群体构建与SSR分子标记鉴定

利用光周期调控技术诱导甘蔗热带种路打士开花，并与滇蔗茅云南95—19进行远缘杂交，培育实生苗76株，大田移栽成活62株，成活率81．6％；选用4对引物对获得的62份杂交后代进行SSR分子标记鉴定，结果表明，62份杂交后代全部为真杂种，所选SSR引物对滇蔗茅后代群体具有较高的鉴定效率。该杂交组合后代杂种真实率为100％，是目前数量最大的滇蔗茅F1群体。...

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Bibliographic Details
Published in植物遗传资源学报 Vol. 13; no. 2; pp. 321 - 324
Main Author 陆鑫 毛钧 刘洪博 刘新龙 马丽 苏火生 蔡青
Format Journal Article
LanguageChinese
Published 云南省农业科学院生物技术与种质资源研究所,昆明650223 2012
云南大学生命科学院,昆明650091
云南省农业科学院甘蔗研究所/云南省甘蔗遗传改良重点实验室,开远,661600%云南省农业科学院甘蔗研究所/云南省甘蔗遗传改良重点实验室,开远661600
Subjects
SSR标记
杂交后代
滇蔗茅
种质创新
种质创新
滇蔗茅
杂交后代
SSR标记
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ISSN1672-1810
DOI10.3969/j.issn.1672-1810.2012.02.028

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Summary:利用光周期调控技术诱导甘蔗热带种路打士开花，并与滇蔗茅云南95—19进行远缘杂交，培育实生苗76株，大田移栽成活62株，成活率81．6％；选用4对引物对获得的62份杂交后代进行SSR分子标记鉴定，结果表明，62份杂交后代全部为真杂种，所选SSR引物对滇蔗茅后代群体具有较高的鉴定效率。该杂交组合后代杂种真实率为100％，是目前数量最大的滇蔗茅F1群体。
Bibliography:LU Xin1, MAO Jun1 , LIU Hong-bo1 , LIU Xin-long1 , MA Li1 , SU Huo-sheng1 , CAI Qing1＇2＇3 （ 1Yunnan Key Laboratory of Sugarcane Genetic Improvement / Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences ,Kaiyuan 661600 ; 2 Biotechnology ＆ Genetic Resources Institute, Yunnan Academy of Agriculture Sciences, Kunming 650223 ; 3 School of Life Sciences, Yunnan University, Kunraing 650091 ）
Erianthus rockii ; Innovation gerplasm ; Hybrid progenies ; SSR marker
Using photoperiod pathway controlling techniques induced S. officinarum Ludashi to bloom, and dis- tant crossing with Erianthus rockii Yunnan 95-19. 62 seedlings have been transplanted and survived in the field. The survival rate was 81.6%. 4 pairs of SSR primers were chosen to use for authenticity identification of progenies. The results showed that all of the 62 progenies were genuine hybrids, and these SSR primers are quite efficient for identification of Erianthus rockii progenies. The genuine hybrids rate of this cross was 100% , and the
ISSN:1672-1810
DOI:10.3969/j.issn.1672-1810.2012.02.028