Necrostatin-1对蛛网膜下腔出血后皮层细胞坏死状凋亡、坏死与自噬的影响

目的研究necrostatin-1对蛛网膜下腔出血（subarachnoidhemorrhage，SAH）后皮层细胞坏死状凋亡、自噬与坏死调节的作用。方法72只SD大鼠随机分成假手术组、模型组、赋形剂组、necrostatin-1组。赋形剂组经侧脑室给予二甲基亚砜2肛L，necrostatin-1组给予necrostatin-15．2肛g溶于2μL二甲基亚砜。血管内穿刺法制作SAH模型。每组9只观察皮质受体相互作用蛋白3和微管相关蛋白1轻链3的变化，检测坏死状凋亡与自噬；9只腹腔注射碘化丙啶观察细胞坏死。对所有大鼠进行6、12、24hLoeffler神经行为学评分。结果Necrostatin-...

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Published in西安交通大学学报（医学版） Vol. 34; no. 6; pp. 740 - 743
Main Author 张斌飞 安吉洋 宋锦宁 张明 罗显华 黄廷钦 翟海程 白立曦
Format Journal Article
LanguageChinese
Published 西安交通大学医学院第一附属医院神经外科,陕西西安,710061 2013
Subjects
necrostatin-1
坏死
坏死状凋亡
大鼠
脑皮质细胞
自噬
蛛网膜下腔出血
自噬
大鼠
蛛网膜下腔出血
坏死
坏死状凋亡
necrostatin-1
脑皮质细胞
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ISSN1671-8259
DOI10.7652/jdyxb201306008

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Summary:目的研究necrostatin-1对蛛网膜下腔出血（subarachnoidhemorrhage，SAH）后皮层细胞坏死状凋亡、自噬与坏死调节的作用。方法72只SD大鼠随机分成假手术组、模型组、赋形剂组、necrostatin-1组。赋形剂组经侧脑室给予二甲基亚砜2肛L，necrostatin-1组给予necrostatin-15．2肛g溶于2μL二甲基亚砜。血管内穿刺法制作SAH模型。每组9只观察皮质受体相互作用蛋白3和微管相关蛋白1轻链3的变化，检测坏死状凋亡与自噬；9只腹腔注射碘化丙啶观察细胞坏死。对所有大鼠进行6、12、24hLoeffler神经行为学评分。结果Necrostatin-1下调皮质受体相互作用蛋白3和微管相关蛋白1轻链3的表达（P〈O．05），减少皮层碘化丙啶阳性细胞数目（P〈0．05），提高12、24h的Loeffler神经功能评分（P〈O．05），有改善SAH预后的作用。结论Necrostatin一1可能在抑制SAH后脑细胞坏死状凋亡时也下调了自噬与坏死水平。
Bibliography:Objective To explore after subarachnoid hemorrhage （SAH） the effects of necrostatin-1 on cortical necroptosis, autophagy and necrosis Methods Totally 72 SD rats were randomly divided into sham operation group, model group, vehicle group and necrostatin-1 group. Rats in vehicle group were given 2 #L of dimethyl sulfoxide by intracerebroventricular injection while those in necrostatin-1 group were given 5.2 ~tg of necrostatin-1 dissolved in 2 ~tL of dimethyl sulfoxide. Subarachnoid hemorrhage models were induced by intravascular puncture. In each group 9 rats were used to observe the expressions of receptor interacting protein 3 and microtubule associated protein 1 light chain 3 in the cerebral cortex by Western blot; 9 rats were injected intraperitoneally with propidium iodide to observe necrosis in each group. Loeffler neurological score was recorded for all rats at 6, 12 and 24 h. Results Necrostatin-1 suppressed receptor interacting protein 3 and microtubule associated protein 1 light chain 3 in the cerebra
ISSN:1671-8259
DOI:10.7652/jdyxb201306008