SOCS1基因沉默对人膀胱癌T24细胞增殖、侵袭及化疗敏感性的影响

目的探讨细胞因子信号转导抑制因子1（SOCS1）基因沉默对人膀胱癌细胞增殖、侵袭及化疗敏感性的影响。方法将培养好的人膀胱癌T24细胞随机分为三组,用Lipo2000将重组质粒p U-SOCS1-siRNA（沉默SOCS1基因）、阴性对照质粒分别转染至干扰组、对照组,空白组不做任何处理。基因沉默后,用RT-PCR技术检测细胞中SOCS1 mRNA表达;Western blotting法检测细胞中SOCS1蛋白表达;MTT法检测细胞增殖率;流式细胞术检测细胞周期及化疗药物诱导的细胞凋亡率;Transwell小室模型检测细胞侵袭能力;MTT法检测细胞化疗敏感性。结果与其他两组比较,干扰组SOCS1基...

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Bibliographic Details
Published in山东医药 Vol. 56; no. 37; pp. 21 - 24
Main Author 闫骏 朱小军 斯钦布和 王国强 陈利刚 黄勇
Format Magazine Article
LanguageChinese
Published 内蒙古医科大学附属医院,呼和浩特,010050 2016
Subjects
化疗敏感性
基因沉默
细胞侵袭
细胞因子信号转导抑制因子1
细胞增殖
膀胱肿瘤
膀胱肿瘤
细胞因子信号转导抑制因子1
基因沉默
suppressor of cytokine signaling protein 1
cell proliferation
human bladder neoplasms
化疗敏感性
gene silencing
cell invasion
chemosensitivity
细胞侵袭
细胞增殖
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ISSN1002-266X
DOI10.3969/j.issn.1002-266X.2016.37.007

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Summary:目的探讨细胞因子信号转导抑制因子1（SOCS1）基因沉默对人膀胱癌细胞增殖、侵袭及化疗敏感性的影响。方法将培养好的人膀胱癌T24细胞随机分为三组,用Lipo2000将重组质粒p U-SOCS1-siRNA（沉默SOCS1基因）、阴性对照质粒分别转染至干扰组、对照组,空白组不做任何处理。基因沉默后,用RT-PCR技术检测细胞中SOCS1 mRNA表达;Western blotting法检测细胞中SOCS1蛋白表达;MTT法检测细胞增殖率;流式细胞术检测细胞周期及化疗药物诱导的细胞凋亡率;Transwell小室模型检测细胞侵袭能力;MTT法检测细胞化疗敏感性。结果与其他两组比较,干扰组SOCS1基因沉默后SOCS1 mRNA和蛋白表达降低（P均〈0.05）;细胞增殖率降低（P均〈0.05）;G0/G1期细胞增多,S、G2/M期细胞减少（P均〈0.05）;吉西他滨诱导的细胞凋亡率升高（P〈0.05）;体外侵袭能力减弱（P均〈0.05）;对化疗药物卡铂和吉西他滨的敏感性增强（P均〈0.05）。结论 SOCS1基因沉默后,人膀胱癌细胞T24的增殖、体外侵袭能力下降,化疗敏感性增强。
Bibliography:37-1156/R
Objective To investigate the effects of suppressor of cytokine signaling-1（ SOCS1） gene silencing on cell proliferation,invasion and chemotherapeutic drugs sensitivity of human bladder cancer cells. Methods Human bladder cancer cell line T24 cells were randomly divided into three groups： the interference group（ which was transfected by recombinant plasmid p U-SOCS1-siRNA）,control group（ which was transfected by negative control plasmid） and blank group without any treatment. RT-PCR and Western blotting were used to detect the SOCS1 mRNA and protein expression in human bladder cancer cell line（ T24） after transfection. The cell proliferation rate was detected by MTT assay,flow cytometry was used to detect the cell cycle and cell apoptosis rate; the invasive ability of T24 cells in vitro was evaluated by Transwell migration assay and the change of chemosensitivity was determined by MTT. Results Compared with the other groups,after silencing SOCS1 in the interference group,the expression levels of SOCS1
ISSN:1002-266X
DOI:10.3969/j.issn.1002-266X.2016.37.007