D101C大孔吸附树脂分离提纯原人参二醇组皂苷研究
文章主要研究人参根总皂苷浓度和吸附温度对D101C大孔吸附树脂选择性吸附原人参二醇组皂苷（PPD）和原人参三醇组皂苷（PPT）影响。HPLC定量分析结果表明，人参根总皂苷浓度和吸附温度对PPD和PPT皂苷吸附影响显著，其中温度对PPD皂苷显示出较高选择性。当浓度为15mg·mL-1。人参根总皂苷溶液用D101C大孔吸附树脂于55℃吸附12h，可吸附378．72mg·g-1 PPD皂苷和54．65mg·g-1 PPT皂苷，经35％和80％乙醇溶液依次解吸，可分离254．94mg·手。PPD皂苷，纯度94．62％。该方法操作简单，产品纯度较高，适用于工业化生产。...
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| Published in | 东北农业大学学报 Vol. 44; no. 6; pp. 90 - 95 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
东北师范大学化学学院,长春,130024%吉林化工学院化学与制药工程学院,吉林吉林,132022
2013
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1005-9369 |
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| Summary: | 文章主要研究人参根总皂苷浓度和吸附温度对D101C大孔吸附树脂选择性吸附原人参二醇组皂苷（PPD）和原人参三醇组皂苷（PPT）影响。HPLC定量分析结果表明，人参根总皂苷浓度和吸附温度对PPD和PPT皂苷吸附影响显著，其中温度对PPD皂苷显示出较高选择性。当浓度为15mg·mL-1。人参根总皂苷溶液用D101C大孔吸附树脂于55℃吸附12h，可吸附378．72mg·g-1 PPD皂苷和54．65mg·g-1 PPT皂苷，经35％和80％乙醇溶液依次解吸，可分离254．94mg·手。PPD皂苷，纯度94．62％。该方法操作简单，产品纯度较高，适用于工业化生产。 |
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| Bibliography: | 23-1391/S SUN Chengpeng, GAO Weiping, ZHAO Baozhong, LI Linling, CHENG Leqin（1. Department of Chemistry, Northeast Normal University, Changchun, 130024, China; 2. Department of Pharmacy and Applied Chemistry, Jilin Institute of Chemical Technology, Jilin Jilin 132022, China） D101C macroporous adsorption resin; temperature; selective adsorption; protopan- axadiol type saponin; protopanaxatriol type saponin The influence of selective adsorption of protopanaxadiol and protopanaxatriol type Saponins was studied by using D101C macroporous adsorption resin, which focused on the tempera- ture and concentration of ginseng root Saponins. The results of HPLC analysis showed that the adsorp- tion capacities of PPD and PPT Saponins were significantly affected by the concentration of ginseng root Saponins and absorption temperature, absorption temperature displayed a highly selectivity for PPD Saponins among them. The solution of ginseng root Saponins （15 mg . mL-1） was adsorbed by using D101C macroporous adsorption resin at |
| ISSN: | 1005-9369 |