参附注射液对肾母细胞瘤细胞增殖、凋亡的影响及机制探讨

目的 观察参附注射液对肾母细胞瘤细胞增殖及凋亡的影响,并探讨其机制。方法 取适量对数生长期肾母细胞瘤细胞,分为低、中、高浓度组及对照组。低、中、高浓度组分别加入0.4、0.8、1.2 mg/m L参附注射液2m L,对照组加入等量无菌DMSO培养液。干预24 h时采用MTT法检测各组细胞增殖抑制率,采用流式细胞仪测算各组细胞凋亡率,采用Western blot法检测各组细胞p53、B细胞淋巴瘤因子l-2（bcl-2）蛋白。结果 干预24 h时低浓度组、中浓度组、高浓度组、对照组的细胞增殖抑制率分别为22.83%±5.18%、35.76%±6.01%、54.51%±9.07%、6.39%±2.8...

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Bibliographic Details
Published in山东医药 Vol. 56; no. 18; pp. 18 - 20
Main Author 仲智勇 时保军 周辉 王文博
Format Magazine Article
LanguageChinese
Published 河北医科大学第二医院,石家庄,050017 2016
Subjects
B细胞淋巴瘤因子-2
p53
参附注射液
细胞凋亡
细胞增殖
肾母细胞瘤
细胞凋亡
B细胞淋巴瘤因子-2
Wilm's tumor
cell proliferation
Shenfu injection
apoptosis
B lymphocyte-2
参附注射液
肾母细胞瘤
细胞增殖
p53
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ISSN1002-266X
DOI10.3969/j.issn.1002-266X.2016.18.006

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Summary:目的 观察参附注射液对肾母细胞瘤细胞增殖及凋亡的影响,并探讨其机制。方法 取适量对数生长期肾母细胞瘤细胞,分为低、中、高浓度组及对照组。低、中、高浓度组分别加入0.4、0.8、1.2 mg/m L参附注射液2m L,对照组加入等量无菌DMSO培养液。干预24 h时采用MTT法检测各组细胞增殖抑制率,采用流式细胞仪测算各组细胞凋亡率,采用Western blot法检测各组细胞p53、B细胞淋巴瘤因子l-2（bcl-2）蛋白。结果 干预24 h时低浓度组、中浓度组、高浓度组、对照组的细胞增殖抑制率分别为22.83%±5.18%、35.76%±6.01%、54.51%±9.07%、6.39%±2.81%,组间比较,P均〈0.05。干预24 h时低浓度组、终浓度组、高浓度组、对照组的细胞凋亡率分别为14.59%±1.24%、18.27%±1.73%、21.85%±1.62%、3.92%±1.36%,组间比较,P均〈0.05。各组细胞p53的相对表达量为高浓度组〈中浓度组〈低浓度组〈对照组,P均〈0.05;bcl-2的相对表达量为高浓度组〉中浓度组〉低浓度组〉对照组,P均〈0.05。结论 参附注射液可抑制肾母细胞瘤细胞增殖,促进细胞凋亡;其机制可能与降低p53表达、升高bcl-2表达有关。
Bibliography:ZHONG Zhiyong, SHI Baojun, ZHOU Hui, WANG Wenbo （The Second Hospital of Hebei Medical University, Shijiazhuang 050017, China）
37-1156/R
Objective To investigate the effects and related mechanism of Shenfu injection on the proliferation and apoptosis of Wiling tumor cells in vitro. Methods Wilm＇s tumor ceils were divided into the control group, low-dose, medi- um-dose and high-dose groups. The low-dose, medium-dose and high-dose groups were treated with 2 ml, of 0.4 mg/mL, 0.8 mg/mL and 1.2 mg/mL Shenfu injection, and the control group was added with DMSO. After 24-hour intervention, the proliferation inhibition rate was detected by MTT, the rate of apoptosis and the expression of p53 and Bcl-2 protein was detected by flow cytometry and Western blotting, respectively. Results After 24-hour intervention, the proliferation inhibi- tion rates of the low-dose group, medium-dose group, high-dose group and the control group were respectively 22.83% ± 5.18% , 35.76% ±6.01%, 54.51% ±9.07% and 6.39% ±2.81%, all P〈0.05. A
ISSN:1002-266X
DOI:10.3969/j.issn.1002-266X.2016.18.006