高糖环境对胃癌组织、胃癌SGC7901细胞株P-糖蛋白表达及5-Fu敏感性的影响

目的探讨高糖环境对胃癌组织、胃癌SGC7901细胞株P-糖蛋白（P-gp）表达及5-氟尿嘧啶（5-Fu）敏感性的影响。方法选择胃癌患者40例、2型糖尿病合并胃癌患者30例,取手术切除的胃癌组织标本,采用免疫组化法观察胃癌组织中P-gp表达情况;取人胃癌SGC7901细胞株,体外培养,并给予1 000、4 500、9 000 mg/L葡萄糖干预（分别设为低、中、高浓度组）。收集各组细胞的总蛋白,Western blotting法检测各组细胞中P-gp表达;MTT法检测各组细胞对5-Fu的敏感性。结果 40例胃癌组织中P-gp的平均光密度值为0.014 6,30例2型糖尿病合并胃癌组织中P-gp的...

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Bibliographic Details
Published in山东医药 Vol. 56; no. 24; pp. 20 - 22
Main Author 张全武 刘芮菡 娄欣
Format Magazine Article
LanguageChinese
Published 郑州大学附属郑州中心医院,郑州,450007 2016
Subjects
5-氟尿嘧啶
P-糖蛋白
胃癌
高糖环境
P-glycoprotein
5-fluorouracil
5-氟尿嘧啶
高糖环境
high glucose
胃癌
P-糖蛋白
gastric carcinoma
Online AccessGet full text
ISSN1002-266X
DOI10.3969/j.issn.1002-266X.2016.24.006

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Summary:目的探讨高糖环境对胃癌组织、胃癌SGC7901细胞株P-糖蛋白（P-gp）表达及5-氟尿嘧啶（5-Fu）敏感性的影响。方法选择胃癌患者40例、2型糖尿病合并胃癌患者30例,取手术切除的胃癌组织标本,采用免疫组化法观察胃癌组织中P-gp表达情况;取人胃癌SGC7901细胞株,体外培养,并给予1 000、4 500、9 000 mg/L葡萄糖干预（分别设为低、中、高浓度组）。收集各组细胞的总蛋白,Western blotting法检测各组细胞中P-gp表达;MTT法检测各组细胞对5-Fu的敏感性。结果 40例胃癌组织中P-gp的平均光密度值为0.014 6,30例2型糖尿病合并胃癌组织中P-gp的平均光密度值为0.021 5,二者比较P〈0.01。高浓度组P-gp的相对表达量为2.92±0.09,中浓度组为2.53±0.09,低浓度组为1.56±0.09,组间两两比较P均〈0.01。随着葡萄糖浓度的升高,5-Fu对胃癌SGC7901细胞的增殖抑制率逐渐下降,尤其以高浓度组效果最明显（P〈0.01）。结论高糖环境可提高胃癌组织及胃癌SGC7901细胞株P-gp表达,从而降低胃癌细胞对5-Fu的敏感性。
Bibliography:ZHANG Quanwu, LIU Ruihan, LOU Xin （Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, China）
37-1156/R
Objective To investigate the effects of high glucose on the expression of P-glycoprotein （P-gp） and 5-Fu sensitivity in gastric cancer tissues and gastric cancer cell line SGC7901. Methods The expression of P-gp from the gas- tric cancer tissues in 40 cases of patients with gastric cancer and 30 case of patients with type 2 diabetes mellitus combined with gastric cancer was detected by immunohistochemistry. Human gastric cancer cell line SGC7901 was cultured and inter- vened by 1 000, 4 500 and 9 000 mg/L glucose （low-dose, medium-dose and high-dose glucose group）. The cell total protein of each group was collected, the expression of P-gp protein in each group was detected by Western blotting, and the sensitivity of cells to 5-Fu was detected by MTF assay. Results The average optical density of P-gp in 40 cases of gastric cancer and 30 case of type 2 diabetes mellitus combined wit
ISSN:1002-266X
DOI:10.3969/j.issn.1002-266X.2016.24.006