Belumosudil reduces oral chronic graft-versus-host disease tissue inflammation and fibrosis: a ROCKstar companion study

• Published in: Blood advances Vol. 9; no. 14; pp. 3479 - 3494
• Main Authors: Sharma, Rubina, Holtzman, Noa G., Pusic, Iskra, Cutler, Corey, Treister, Nathaniel, Mehta, Rohtesh S., Alousi, Amin S., Vigneswaran, Nadarajah, Javaid, Ayesha, Boksa, Francis, Mody, Drashty P., Costa-da-Silva, Ana C., Schueller, Olivier, Macé, Sandrine, Yao, Yu, Ji, Ran, Hu, Beifang, Marshall, Kathy, Blazar, Bruce R., Lee, Stephanie J., Pavletic, Steven Z., Mays, Jacqueline W.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 22.07.2025; The American Society of Hematology; Elsevier
• Subjects: Adult; Aged; Chronic Disease; Female; Fibrosis - drug therapy; Graft vs Host Disease - drug therapy; Graft vs Host Disease - etiology; Graft vs Host Disease - metabolism; Graft vs Host Disease - pathology; Hematology; Humans; Inflammation - drug therapy; Inflammation - etiology; Inflammation - pathology; Interleukin-17 - metabolism; Male; Middle Aged; Mouth Mucosa - drug effects; Mouth Mucosa - metabolism; Mouth Mucosa - pathology; Protein Kinase Inhibitors - therapeutic use; rho-Associated Kinases - antagonists & inhibitors; Transplantation
• ISSN: 2473-9529; 2473-9537; 2473-9537
• DOI: 10.1182/bloodadvances.2025016170

•Belumosudil treatment for cGVHD decreased collagen content in the OM.•Belumosudil reduced IL-17 production and inflammation in the OM and MSGs in oral cGVHD. [Display omitted] Belumosudil (KD025), an oral, selective, Rho-associated, coiled-coil–containing protein kinase 2 (ROCK2) inhibitor, is approved for third-line treatment of chronic graft-versus-host disease (cGVHD). Previous studies demonstrated that ROCK2 inhibition reduces blood interleukin-17 (IL-17) activity and promotes regulatory T-cell (Treg cell) recovery. However, these studies did not evaluate immune responses within cGVHD-affected tissues. This study assessed tissue-level immune dynamics in 20 patients with oral cGVHD from the phase 2 ROCKstar trial, before and after 6 months of belumosudil treatment, focusing on key effector sites (oral mucosa [OM], minor salivary glands [MSGs], and skin) and the peripheral blood. After belumosudil treatment, reduction in collagen was observed in OM in parallel with decreased IL-17+ cell frequency in both OM (n = 14 pairs) and MSG (n = 11 pairs). IL-17 was primarily produced by non–T cells in the oral tissues. Immune cell frequencies in the OM decreased after treatment, whereas CD4 Treg cells increased in both the MSG and blood. Per overall or mouth-specific clinical response criteria, responders to belumosudil exhibited a reduction in collagen type I and IL-17 in the OM. Additionally, salivary transforming growth factor β1 (TGF-β1), a critical driver of fibrosis, decreased significantly, with a strong correlation observed between TGF-β1 and IL-17 levels. These findings illustrate the tissue-level response to belumosudil therapy and suggest that there is a reduction in tissue fibrosis and inflammation, thereby highlighting the therapeutic impact of ROCK2 inhibition in mitigating cGVHD. The ROCKstar study was registered at www.ClinicalTrials.gov as #NCT03640481.