番茄抗叶霉病基因Cf-10和Cf-16的遗传分析及SSR标记

以Cf-10和Cf-16基因对东北三省分离鉴定的8个番茄叶霉病病原茵生理小种的抗性进行了评价，表明Cf-10和Cf-16基因对分化出的生理小种具有良好的抗性。分别对07881x08HN30（含Cf-10）和07880x08HN34（含Cf-16）构建的F1、F2代分离群体进行了遗传分析，并对F2群体进行了SSR标记。遗传分析表明，08HN30和08HN34的抗性均由一对显性单基因控制，从341对SSR引物中筛选出2个与Cf-10基因连锁的标记LEtaa001和LEaat003，遗传距离分别为9．7和22．9cM；1个与Cf-16基因连锁的标记Toml44—145，遗传距离为10．4cM。上述研...

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Published in东北农业大学学报 Vol. 43; no. 1; pp. 88 - 92
Main Author 李宁 许向阳 姜景彬 李景富
Format Journal Article
LanguageChinese
Published 湖北省农业科学院经济作物研究所,武汉,430064%东北农业大学园艺学院,哈尔滨,150030 2012
Subjects
cf-10
Cf-16
sSR标记
叶霉病
番茄
Cf-16
番茄
Cf-10
SSR标记
叶霉病
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ISSN1005-9369
DOI10.3969/j.issn.1005-9369.2012.01.015

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Summary:以Cf-10和Cf-16基因对东北三省分离鉴定的8个番茄叶霉病病原茵生理小种的抗性进行了评价，表明Cf-10和Cf-16基因对分化出的生理小种具有良好的抗性。分别对07881x08HN30（含Cf-10）和07880x08HN34（含Cf-16）构建的F1、F2代分离群体进行了遗传分析，并对F2群体进行了SSR标记。遗传分析表明，08HN30和08HN34的抗性均由一对显性单基因控制，从341对SSR引物中筛选出2个与Cf-10基因连锁的标记LEtaa001和LEaat003，遗传距离分别为9．7和22．9cM；1个与Cf-16基因连锁的标记Toml44—145，遗传距离为10．4cM。上述研究结果为抗源基因在番茄抗叶霉病育种中的应用及进一步精细定位Cf-10和Cf-16基因奠定了基础。
Bibliography:LI Ning, XU Xiangyang, JIANG Jingbin, LI Jingfu （1. Cash Crop Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China; 2. College of Horticulture, Northeast Agricultural University, Harbin 150030, China）
tomato; Cf-10; Cf-16; leaf mould disease; SSR marker
23-1391/S
Tomato Cf-10 and Cf-16 gene were resistant to eight prevalent physiological races of C. fulvum present in three northeastern provinces of China by virulence tests. FI and F2 populations were developed from 07881× 08HN30 and 07880 × 08HN34. The inheritance analysis indicated that leaf mould resistance gene Cf-10 and Cf-16 were single dominant genes, respectively. Total 341 pair of SSR primers were used to identify molecular markers linked to Cf-10 and Cf-16 gene, two polymorphic markers LEtaa001and LEaat003 were identified to be linked to Cf-10 in 08HN30 with the genetic distances of 9.7 and 22.9 cM, respectively, while a SSR marker Tom144-145 located on chromosome 11 were linked to Cf-16 resistant gene with the genetic distanc
ISSN:1005-9369
DOI:10.3969/j.issn.1005-9369.2012.01.015