利用SSR标记分析水稻抗瘟材料的遗传多样性

选用分布于水稻12条染色体上与抗稻瘟病基因紧密连锁的86对SSR引物，对30份稻瘟病抗性水稻材料进行遗传多样性分析。结果表明，19对引物在30份材料间表现出多态性。UPGMA聚类分析结果表明，供试材料可分为6类，反映出供试材料抗性遗传相似度低，遗传背景差异较大。聚类结果与材料的田间抗性表现基本一致，遗传亲缘关系基本吻合，说明利用SSR标记分析水稻品种资源遗传基础具有一定的可靠性。试验为供试材料遗传多样性的研究利用和抗瘟新品种的选育提供了理论依据。...

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Bibliographic Details
Published in东北农业大学学报 Vol. 42; no. 10; pp. 20 - 25
Main Author 邱琳 李俊刚 陈英 涂升斌
Format Journal Article
LanguageChinese
Published 中国科学院成都生物研究所,成都,610041%仁寿县石咀乡农业服务中心,四川眉山,620575 2011
Subjects
SSR分子标记
水稻抗瘟材料
聚类分析
遗传多样性
水稻抗瘟材料
遗传多样性
SSR分子标记
聚类分析
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ISSN1005-9369
DOI10.3969/j.issn.1005-9369.2011.10.005

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Summary:选用分布于水稻12条染色体上与抗稻瘟病基因紧密连锁的86对SSR引物，对30份稻瘟病抗性水稻材料进行遗传多样性分析。结果表明，19对引物在30份材料间表现出多态性。UPGMA聚类分析结果表明，供试材料可分为6类，反映出供试材料抗性遗传相似度低，遗传背景差异较大。聚类结果与材料的田间抗性表现基本一致，遗传亲缘关系基本吻合，说明利用SSR标记分析水稻品种资源遗传基础具有一定的可靠性。试验为供试材料遗传多样性的研究利用和抗瘟新品种的选育提供了理论依据。
Bibliography:QIU Lin, LI Jungang, CHEN Ying, TU Shengbin（l. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; 2. Agricultural Service Center of Shiju Village in Renshou County, Meishan Sichuan 620575, China）
23-1391/S
Total 86 SSR primers located at 12 chromosomes in rice, which were closely linked with blast resistance genes, were selected to analyze the genetic diversity of 30 blast resistant rice materials. The results indicated that 19 SSR markers performed polymorphism in the 30 materials. The results of cluster analysis by UPGMA method showed that 30 materials could be clustered into six groups which reflected that those tested materials had low similarity in resistance inheritance and large variation in genetic background. The field resistance performance of those materials was basically consistent with the results of cluster analysis and genetic relationship was basically coincided, which showed that it was reliable to use SSR markers to analyze genetic basis of rice. The experiment p
ISSN:1005-9369
DOI:10.3969/j.issn.1005-9369.2011.10.005