Orexin-A对大鼠海马神经细胞凋亡的影响及机制

目的探讨Orexin-A对大鼠海马神经细胞凋亡的影响及作用机制。方法提取36只Wistar大鼠的海马神经细胞,培养后随机分为空病毒组、PLCβ1组、PLCβ4组,分别用空病毒和携带目的基因PLCβ1、PLCβ4的慢病毒转染沉默目的基因,各组各自取一半细胞加入Orexin-A（100 nmol/L）培养8 h,用流式细胞术测算各组细胞凋亡指数,Western blotting法检测海马神经细胞外信号调节激酶（ERK）1/2的表达。结果与不加Orexin-A比较,加Orexin-A后空病毒组、PLCβ4组海马神经细胞凋亡率降低、ERK1/2表达升高（P均〈0.05）,而PLCβ1组海马神经细胞凋亡...

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Bibliographic Details
Published in山东医药 Vol. 56; no. 9; pp. 10 - 12
Main Author 池恒 李国华 唐吉友
Format Magazine Article
LanguageChinese
Published 山东省千佛山医院,济南250014 2016
泰山医学院%济南市第三人民医院%山东省千佛山医院,济南,250014
Subjects
Orexin-A
大鼠
海马神经细胞
细胞凋亡
细胞外信号调节激酶1/2
细胞凋亡
hippocampal nerve cells
细胞外信号调节激酶1/2
大鼠
Orexin-A
海马神经细胞
apoptosis
rats
extracellular signal-regulated kinase 1/2
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ISSN1002-266X
DOI10.3969/j.issn.1002-266X.2016.09.004

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Summary:目的探讨Orexin-A对大鼠海马神经细胞凋亡的影响及作用机制。方法提取36只Wistar大鼠的海马神经细胞,培养后随机分为空病毒组、PLCβ1组、PLCβ4组,分别用空病毒和携带目的基因PLCβ1、PLCβ4的慢病毒转染沉默目的基因,各组各自取一半细胞加入Orexin-A（100 nmol/L）培养8 h,用流式细胞术测算各组细胞凋亡指数,Western blotting法检测海马神经细胞外信号调节激酶（ERK）1/2的表达。结果与不加Orexin-A比较,加Orexin-A后空病毒组、PLCβ4组海马神经细胞凋亡率降低、ERK1/2表达升高（P均〈0.05）,而PLCβ1组海马神经细胞凋亡率、ERK1/2表达无明显变化（P均〉0.05）。结论 Orexin-A可减少大鼠海马神经细胞的凋亡,该作用可能与OX1R/OX2R-Gq-PLCβ1信号通路有关。
Bibliography:CHI Heng , LI Guohua, TANG Jiyou （1 Shandong Provincial Qianfoshan Hospital, Jinan 250014, China）
37-1156/R
Orexin-A; hippocampal nerve cells; apoptosis; extracellular signal-regulated kinase 1 /2; rats
Objective To invesitage the effect of Orexin-A on the apoptosis of rat hippocampal nerve cells and the mechanism of action. Methods The primary cultured hippocampal nerve cells from 36 Wistar rats were randomly divided in the empty virus group,PLCβ1group and PLCβ4group which were respectively transfected by empty vectors,lentiviruses with PLCβ1and lentiviruses with PLCβ4. After the transfection,all three groups were further divided into subgroups treated with Orexin-A respectively for 8 h. We investigated the expression of ERK1 /2by Western blotting and apoptosis rate was measured by flow cytometry for each subgroup. Results After treatment of Orexin-A,the expression of ERK1 /2was increased and the apoptosis rate was decreased significantly in the PLCβ4group and empty virus group（ all P〈0. 05）. However,these chan
ISSN:1002-266X
DOI:10.3969/j.issn.1002-266X.2016.09.004