高脂饮食诱导非酒精性脂肪性肝病小鼠模型肝脏microRNA表达谱的变化分析

目的研究microRNA（miRNA）-384的表达对高脂饮食诱导非酒精性脂肪性肝病（NAFLD）小鼠肝脂肪变性的影响。方法将30只雄性C57BL/6J小鼠适应性喂养7 d,然后随机分成2组,每组15只。对照组中的15只小鼠给予普通饮食,模型组15只小鼠给予HFD,喂养8周后获取肝组织。进行HE和尼罗红染色观察肝组织病理改变。采用微阵序列测序肝组织的全基因组miRNA表达谱,PCR法测定miRNA-384的相对表达水平。计量资料2组间比较采用t检验。结果对照组小鼠肝脏呈红色,边缘锐利,肝小叶结构清晰,肝细胞无脂肪变性;模型组小鼠肝脏呈黄色,边缘变钝,肝细胞明显肿胀,细胞质内可见大量脂肪空泡,脂...

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Bibliographic Details
Published in临床肝胆病杂志 Vol. 33; no. 12; pp. 2372 - 2375
Main Author 麦静愔;陈天阳;成扬
Format Journal Article
LanguageChinese
Published 上海浦东新区中医医院,上海,201299%上海中医药大学附属曙光医院,上海,201203 2017
Subjects
基因表达
小鼠,近交C57BL
微RNAs
非酒精性脂肪性肝病
高脂饮食
microRNAs
inbred C578BL
非酒精性脂肪性肝病
高脂饮食
nonalcoholic fatty liver disease
基因表达
小鼠,近交C57BL
mice
gene expression
high fat diet
微RNAs
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ISSN1001-5256
DOI10.3969/j.issn.1001-5256.2017.12.023

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Summary:目的研究microRNA（miRNA）-384的表达对高脂饮食诱导非酒精性脂肪性肝病（NAFLD）小鼠肝脂肪变性的影响。方法将30只雄性C57BL/6J小鼠适应性喂养7 d,然后随机分成2组,每组15只。对照组中的15只小鼠给予普通饮食,模型组15只小鼠给予HFD,喂养8周后获取肝组织。进行HE和尼罗红染色观察肝组织病理改变。采用微阵序列测序肝组织的全基因组miRNA表达谱,PCR法测定miRNA-384的相对表达水平。计量资料2组间比较采用t检验。结果对照组小鼠肝脏呈红色,边缘锐利,肝小叶结构清晰,肝细胞无脂肪变性;模型组小鼠肝脏呈黄色,边缘变钝,肝细胞明显肿胀,细胞质内可见大量脂肪空泡,脂滴相互融合导致细胞核偏位。与正常小鼠相比,在NAFLD小鼠的肝组织中发现了12个上调和18个下调的miRNA。筛查了对照组和模型组间的一些差异表达的miRNA,获得同样的聚类分析图。在这8个明显改变的miRNA中,miRNA-384具有显著的倍数变化。结论 miRNA-384表达的上调与肝脏脂肪变性有着密切关系,但是其作用机制仍需进一步研究。
Bibliography:Objective To investigate the effect of miRNA-384（ miR-384） expression on hepatic steatosis in mice with nonalcoholic fatty liver disease（ NAFLD） induced by high-fat diet（ HFD）. Methods A total of 30 male C57 BL/6 J mice were fed for 7 days to adapt to the environment and then randomly divided into 2 groups,with 15 mice in each group. The mice in the control group were given normal diet,and those in the model group were given HFD for 8 weeks and then the liver tissue was harvested. HE and Nile red staining were used to observe the pathological changes of the liver. Microarray sequencing was performed to determine the whole-genome miRNA expression profile of liver tissue,and PCR was used to measure the relative expression of miR-384. The t-test was used for the comparison of continuous data between groups. Results In the control group,the liver was red with sharp edges,the lobular structure was clear,and there was no hepatic steatosis; in the model group,the liver was yellow with blunt edges,and the hepatocytes
ISSN:1001-5256
DOI:10.3969/j.issn.1001-5256.2017.12.023