烟草根际放线菌的分离及酶活性和功能基因检测

从云南、贵州、河南等地采集29份烟草健田土壤样品，采用4种土壤预处理方法及4种培养基分离得到607株放线菌。利用平板透明圈法对42株代表性放线茵进行了蛋白酶、几丁质酶、纤维素酶和淀粉酶活性检测；采用特异性引物扩增法，筛选其聚酮合酶（PKS—I、PKS-Ⅱ）基因和非核糖体多肽合成酶（NRPS）基因。对4株菌进行16SrDNA初步分类鉴定。结果表明，供试42株放线菌中四种酶活性的初筛阳性率为54．8％-90．5％；其中4株放线菌含三种化合物合成基因，16SrRNA序列测定4株菌均属链霉菌。...

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Published in东北农业大学学报 Vol. 45; no. 2; pp. 19 - 23
Main Author 曹毅 陆宁 陈兴江 郭玉双 夏海乾 商胜华
Format Journal Article
LanguageChinese
Published 贵州省烟草科学研究院,贵阳,550081 2014
Subjects
化合物合成基因
放线菌
根际
烟草
酶活性
放线菌
根际
actinomycete
化合物合成基因
烟草
enzyme activity
tobacco
synthesis gene of secondary metabolite
酶活性
rhizosphere
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ISSN1005-9369

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Summary:从云南、贵州、河南等地采集29份烟草健田土壤样品，采用4种土壤预处理方法及4种培养基分离得到607株放线菌。利用平板透明圈法对42株代表性放线茵进行了蛋白酶、几丁质酶、纤维素酶和淀粉酶活性检测；采用特异性引物扩增法，筛选其聚酮合酶（PKS—I、PKS-Ⅱ）基因和非核糖体多肽合成酶（NRPS）基因。对4株菌进行16SrDNA初步分类鉴定。结果表明，供试42株放线菌中四种酶活性的初筛阳性率为54．8％-90．5％；其中4株放线菌含三种化合物合成基因，16SrRNA序列测定4株菌均属链霉菌。
Bibliography:23-1391/S
tobacco; rhizosphere; actinomycete; enzyme activity; synthesis gene of secondarymetabolite
CAO Yi, LU Ning, CHEN Xingjiang, GUO Yushuang, XlA Haiqian, SHANG Shenghua（Guizhou Academy of Tobacco Science, Guiyang 550081, China）
Using 4 soil pretreatment method and 4 medium, we isolated 607 strains of actinomycetes from 29 tobacco rhizosphere soil samples collected from Yunnan, Guizhou and Henan. The protease, chitinase, cellulase and amylase activity of 42 representative strains were tested byclarity circle method on plate. Genes encoding type land II polyketide synthases（ PKS-I, PKS-II）, nondbosomal peptide synthase （NRPS） were screened by PCR. Four isolates were preliminary classified by its 16S rRNA gene sequences. The 42 tested strains of actinomycetes showed different enzyme activities. There were four strains showed positive results for PKS （type land II ） and NRPS genes screening, the four strains belongs to Streptomyces according to 16S rRNA sequence analysis.
ISSN:1005-9369