MicroRNA-200c and microRNA- 141 are regulated by a FOXP3-KAT2B axis and associated with tumor metastasis in breast cancer

• Published in: Breast cancer research : BCR Vol. 19; no. 1; pp. 73 - 13
• Main Authors: Zhang, Guangxin, Zhang, Wei, Li, Bingjin, Stringer-Reasor, Erica, Chu, Chengjing, Sun, Liyan, Bae, Sejong, Chen, Dongquan, Wei, Shi, Jiao, Kenneth, Yang, Wei-Hsiung, Cui, Ranji, Liu, Runhua, Wang, Lizhong
• Format: Journal Article
• Language: English
• Published: London BioMed Central 21.06.2017; BioMed Central Ltd; BMC
• Subjects: Adult; Aged; Animals; Benign; Biomarkers; Biomarkers, Tumor; Biomedical and Life Sciences; Biomedicine; Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cancer metastasis; Cancer Research; Cell Line, Tumor; Circulating MicroRNA; Circulation; Development and progression; Disease Models, Animal; Disease Progression; Epithelial cells; Exosome; Female; Forkhead Transcription Factors - genetics; FOXP3; Foxp3 protein; Gene expression; Gene Expression Regulation, Neoplastic; Genetic aspects; Human subjects; Humans; Lungs; Lymphatic system; Metastases; Metastasis; Mice; MicroRNA; MicroRNAs; MicroRNAs - genetics; Middle Aged; miRNA; Mutation; Neoplasm Grading; Neoplasm Metastasis; Neoplasm Staging; Oncology; p300-CBP Transcription Factors - genetics; Plasma levels; Research Article; ROC Curve; Rodents; Surgical Oncology; Transcription, Genetic; Tumor cells; Tumor metastasis; Tumors; Womens health; United States > US; microRNA; Breast cancer; Exosome; FOXP3; Circulation; Tumor metastasis
• ISSN: 1465-542X; 1465-5411; 1465-542X
• DOI: 10.1186/s13058-017-0858-x

Background Members of the microRNA (miR)-200 family, which are involved in tumor metastasis, have potential as cancer biomarkers, but their regulatory mechanisms remain elusive. Methods We investigated FOXP3 -inducible breast cancer cells, Foxp3 heterozygous Scurfy mutant ( Foxp3 sf/+ ) female mice, and patients with breast cancer for characterization of the formation and regulation of the miR-200 family in breast cancer cells and circulation. Participants (259), including patients with breast cancer or benign breast tumors, members of breast cancer families, and healthy controls, were assessed for tumor and circulating levels of the miR-200 family. Results First, we identified a FOXP3-KAT2B-miR-200c/141 axis in breast cancer cells. Second, aging Foxp3 sf/+ female mice developed spontaneous breast cancers and lung metastases. Levels of miR-200c and miR-141 were lower in Foxp3 sf/+ tumor cells than in normal breast epithelial cells, but plasma levels of miR-200c and miR-141 in the Foxp3 sf/+ mice increased during tumor progression and metastasis. Third, in patients with breast cancer, the levels of miR-200c and 141 were lower in FOXP3 low relative to those with FOXP3 high breast cancer cells, especially in late-stage and metastatic cancer cells. The levels of miR-200c and miR-141 were higher in plasma from patients with metastatic breast cancer than in plasma from those with localized breast cancer, with benign breast tumors, with a family history of breast cancer, or from healthy controls. Finally, in Foxp3 sf/+ mice, plasma miR-200c and miR-141 appeared to be released from tumor cells. Conclusions miR-200c and miR-141 are regulated by a FOXP3-KAT2B axis in breast cancer cells, and circulating levels of miR-200c and miR-141 are potential biomarkers for early detection of breast cancer metastases.