Identifying mislabeled and contaminated DNA methylation microarray data: an extended quality control toolset with examples from GEO

• Published in: Clinical epigenetics Vol. 10; no. 1; pp. 73 - 9
• Main Authors: Heiss, Jonathan A., Just, Allan C.
• Format: Journal Article
• Language: English
• Published: London BioMed Central 01.06.2018; BioMed Central Ltd; Springer Nature B.V; BMC
• Subjects: 450K; All other subjects; Analysis; Biomedical and Life Sciences; Biomedicine; Consortia; Contamination; CpG Islands; Data processing; Databases, Genetic - standards; Datasets; Deoxyribonucleic acid; DNA; DNA Methylation; DNA microarrays; DNA probes; EPIC; Epigenetics; Epigenomics; Epigenomics - standards; Fingerprinting; Gene expression; Gene Function; Genome, Human; Genomes; Genomics; Human Genetics; Humans; Infinium; Metadata; Methodology; Methylation; Oligonucleotide Array Sequence Analysis - standards; Polymorphism, Single Nucleotide; Quality Control; Sex; Single-nucleotide polymorphism; Software; Statistical analysis; Epigenomics; 450K; EPIC; Data cleaning; Infinium; Quality control; DNA methylation; Mislabeling; Contamination
• ISSN: 1868-7075; 1868-7083; 1868-7083; 1868-7075
• DOI: 10.1186/s13148-018-0504-1

Background Mislabeled, contaminated or poorly performing samples can threaten power in methylation microarray analyses or even result in spurious associations. We describe a set of quality checks for the popular Illumina 450K and EPIC microarrays to identify problematic samples and demonstrate their application in publicly available datasets. Methods Quality checks implemented here include 17 control metrics defined by the manufacturer, a sex check to detect mislabeled sex-discordant samples, and both an identity check for fingerprinting sample donors and a measure of sample contamination based on probes querying high-frequency SNPs. These checks were tested on 80 datasets comprising 8327 samples run on the 450K microarray from the GEO repository. Results Nine hundred forty samples were flagged by at least one control metric and 133 samples from 20 datasets were assigned the wrong sex. In a dataset in which a subset of samples appear contaminated with a single source of DNA, we demonstrate that our measure based on outliers among SNP probes was strongly correlated (> 0.95) with another independent measure of contamination. Conclusions A more complete examination of samples that may be mislabeled, contaminated, or have poor performance due to technical problems will improve downstream analyses and replication of findings. We demonstrate that quality control problems are prevalent in a public repository of DNA methylation data. We advocate for a more thorough quality control workflow in epigenome-wide association studies and provide a software package to perform the checks described in this work. Reproducible code and supplementary material are available at https://doi.org/10.5281/zenodo.1172730 .