VEGF基因多态性及吸烟在诱发非酒精性脂肪肝中的交互作用

目的观察非酒精性脂肪肝（NAFLD）患者血管内皮生长因子（VEGF）基因rs833061、rs3025039位点多态性,并探讨其多态性及吸烟在诱发NAFLD中的交互作用。方法选择NAFLD患者341例（NAFLD组）、健康体检者246例（对照组）,采用PCR-RFLP法分析VEGF基因rs833061、rs3025039位点基因型,非条件Logistic回归分析法分析各基因型与NAFLD易感性的关系,非条件Logistic回归分析法和广义多因子降维法分析VEGF基因多态性及吸烟在诱发NAFLD中的交互作用。结果 VEGF基因rs833061位点C基因携带者（CC＋CT）罹患NAFLD的风险较非...

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Bibliographic Details
Published in山东医药 Vol. 56; no. 8; pp. 7 - 9
Main Author 王倩 吴鹏波 李明 罗和生 余媛洁 谭诗云 舒泳翔
Format Magazine Article
LanguageChinese
Published 武汉大学人民医院,武汉,430060 2016
Subjects
交互作用
基因多态性
易感性
血管内皮生长因子
非酒精性脂肪肝
易感性
基因多态性
non-alcoholic fatty liver disease
susceptibility
非酒精性脂肪肝
vascular endothelial growth factor
interaction
交互作用
血管内皮生长因子
gene polymorphisms
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ISSN1002-266X
DOI10.3969/j.issn.1002-266X.2016.08.003

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Summary:目的观察非酒精性脂肪肝（NAFLD）患者血管内皮生长因子（VEGF）基因rs833061、rs3025039位点多态性,并探讨其多态性及吸烟在诱发NAFLD中的交互作用。方法选择NAFLD患者341例（NAFLD组）、健康体检者246例（对照组）,采用PCR-RFLP法分析VEGF基因rs833061、rs3025039位点基因型,非条件Logistic回归分析法分析各基因型与NAFLD易感性的关系,非条件Logistic回归分析法和广义多因子降维法分析VEGF基因多态性及吸烟在诱发NAFLD中的交互作用。结果 VEGF基因rs833061位点C基因携带者（CC＋CT）罹患NAFLD的风险较非C基因携带者（TT）升高（P均〈0.01）,rs3025039位点T基因携带者（TT＋CT）患病风险较非T基因携带者（CC）风险升高（P均〈0.01）。VEGF基因rs833061位点多态性与吸烟可能在NAFLD发病过程中存在相加作用（P〈0.01）,rs833061位点CC/CT基因型吸烟个体罹患NAFLD风险是携带TT基因型非吸烟个体的4.93倍。结论VEGF基因多态性与NAFLD发病有关,VEGF基因rs833061位点多态性与吸烟在诱发NAFLD过程中具有协同效应。
Bibliography:37-1156/R
non-alcoholic fatty liver disease; vascular endothelial growth factor; gene polymorphisms; susceptibility; interaction
Objective To observe the gene polymorphisms of vascular endothelial growth factor（ VEGF） rs833061 and rs3025039 and to investigate their interaction with smoking in patients with non-alcoholic fatty liver disease（ NAFLD）.Methods Genotypes of VEGF rs833061 and rs3025039 in 341 patients with NAFLD（ NAFLD group） and 246 control subjects（ control group） were examined by polymerase chain reaction-based restriction fragment length polymorphism（ PCR-RFLP）. The unconditional logistic regression（ ULR） was performed to analyze the relationships between the genotypes and susceptibility to NAFLD. The interactions between genotypes of VEGF rs833061 and rs3025039 and smoking in inducing NAFLD were explored by generalized multifactor dimensionality reduction（ GMDR） and unconditional Logistic regression method. Results The risk of NAFLD in VEGF rs833061 with genotype C（ CC ＋ CT） was higher than that
ISSN:1002-266X
DOI:10.3969/j.issn.1002-266X.2016.08.003