Preparation of Monoclonal Antibodies for Immunoblotting Human Growth Hormone Receptor and Growth Hormone-Binding Protein
Monoclonal anti-peptide antibodies against the extracellular domain of human growth hormone receptor (hGHR) were prepared. Four monoclonal antibodies (mAbs) reacted with an extracellular domain protein produced by genetic engineering. Among them, GHRP2-88 was the most reactive against hGHRs from hum...
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| Published in | Biological & pharmaceutical bulletin Vol. 17; no. 7; pp. 983 - 986 |
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| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Tokyo
The Pharmaceutical Society of Japan
1994
Maruzen Japan Science and Technology Agency |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0918-6158 1347-5215 1347-5215 |
| DOI | 10.1248/bpb.17.983 |
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| Abstract | Monoclonal anti-peptide antibodies against the extracellular domain of human growth hormone receptor (hGHR) were prepared. Four monoclonal antibodies (mAbs) reacted with an extracellular domain protein produced by genetic engineering. Among them, GHRP2-88 was the most reactive against hGHRs from human IM-9 cells. The lower limit of detection for immunoblotting using this mAb was about 200 pg hGHR. The GHRP2-88 antibody also reacted with deglycosylated hGHRs from tunicamycin-treated IM-9 cells and with the growth hormone-binding protein (GH-BP) in human plasma. |
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| AbstractList | Monoclonal anti-peptide antibodies against the extracellular domain of human growth hormone receptor (hGHR) were prepared. Four monoclonal antibodies (mAbs) reacted with an extracellular domain protein produced by genetic engineering. Among them, GHRP2-88 was the most reactive against hGHRs from human IM-9 cells. The lower limit of detection for immunoblotting using this mAb was about 200 pg hGHR. The GHRP2-88 antibody also reacted with deglycosylated hGHRs from tunicamycin-treated IM-9 cells and with the growth hormone-binding protein (GH-BP) in human plasma.Monoclonal anti-peptide antibodies against the extracellular domain of human growth hormone receptor (hGHR) were prepared. Four monoclonal antibodies (mAbs) reacted with an extracellular domain protein produced by genetic engineering. Among them, GHRP2-88 was the most reactive against hGHRs from human IM-9 cells. The lower limit of detection for immunoblotting using this mAb was about 200 pg hGHR. The GHRP2-88 antibody also reacted with deglycosylated hGHRs from tunicamycin-treated IM-9 cells and with the growth hormone-binding protein (GH-BP) in human plasma. Monoclonal anti-peptide antibodies against the extracellular domain of human growth hormone receptor (hGHR) were prepared. Four monoclonal antibodies (mAbs) reacted with an extracellular domain protein produced by genetic engineering. Among them, GHRP2-88 was the most reactive against hGHRs from human IM-9 cells. The lower limit of detection for immunoblotting using this mAb was about 200 pg hGHR. The GHRP2-88 antibody also reacted with deglycosylated hGHRs from tunicamycin-treated IM-9 cells and with the growth hormone-binding protein (GH-BP) in human plasma. |
| Author | IKEBUCHI, Hideharu SAWADA, Junichi ASAKURA, Akira YAMAZAKI, Takeshi SUZUKI, Kazuhiro SAITO, Yoshiro TERAO, Tadao OTA, Yoshimi |
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| SubjectTerms | Antibodies, Monoclonal - immunology Biological and medical sciences Carrier Proteins - analysis Carrier Proteins - immunology Cell Line Fundamental and applied biological sciences. Psychology growth hormone receptor growth hormone-binding protein Hormones and neuropeptides. Regulation Humans Hypothalamus. Hypophysis. Epiphysis. Urophysis Immunoblotting monoclonal antibody Receptors, Somatotropin - analysis Receptors, Somatotropin - immunology Vertebrates: endocrinology |
| Title | Preparation of Monoclonal Antibodies for Immunoblotting Human Growth Hormone Receptor and Growth Hormone-Binding Protein |
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