Preparation of Monoclonal Antibodies for Immunoblotting Human Growth Hormone Receptor and Growth Hormone-Binding Protein

• Published in: Biological & pharmaceutical bulletin Vol. 17; no. 7; pp. 983 - 986
• Main Authors: TERAO, Tadao, ASAKURA, Akira, SAWADA, Junichi, SAITO, Yoshiro, IKEBUCHI, Hideharu, OTA, Yoshimi, YAMAZAKI, Takeshi, SUZUKI, Kazuhiro
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 1994; Maruzen; Japan Science and Technology Agency
• Subjects: Antibodies, Monoclonal - immunology; Biological and medical sciences; Carrier Proteins - analysis; Carrier Proteins - immunology; Cell Line; Fundamental and applied biological sciences. Psychology; growth hormone receptor; growth hormone-binding protein; Hormones and neuropeptides. Regulation; Humans; Hypothalamus. Hypophysis. Epiphysis. Urophysis; Immunoblotting; monoclonal antibody; Receptors, Somatotropin - analysis; Receptors, Somatotropin - immunology; Vertebrates: endocrinology; Protein hormone; Binding protein; Signal transduction; Adenohypophyseal hormone; Investigation method; STH; Immunoblotting assay; Monoclonal antibody; Mechanism of action; Hormonal receptor
• ISSN: 0918-6158; 1347-5215; 1347-5215
• DOI: 10.1248/bpb.17.983

Monoclonal anti-peptide antibodies against the extracellular domain of human growth hormone receptor (hGHR) were prepared. Four monoclonal antibodies (mAbs) reacted with an extracellular domain protein produced by genetic engineering. Among them, GHRP2-88 was the most reactive against hGHRs from human IM-9 cells. The lower limit of detection for immunoblotting using this mAb was about 200 pg hGHR. The GHRP2-88 antibody also reacted with deglycosylated hGHRs from tunicamycin-treated IM-9 cells and with the growth hormone-binding protein (GH-BP) in human plasma.