Identification and characterization of pseudogenes in the rice gene complement

• Published in: BMC genomics Vol. 10; no. 1; p. 317
• Main Authors: Thibaud-Nissen, Françoise, Ouyang, Shu, Buell, C Robin
• Format: Journal Article
• Language: English
• Published: London BioMed Central 16.07.2009; BioMed Central Ltd; BMC
• Subjects: Animal Genetics and Genomics; Automation; Biomedical and Life Sciences; DNA, Plant - genetics; Gene expression; Genes, Plant; Genetic aspects; Genetics; Genome, Plant; Genomes; Genomics; Life Sciences; Microarrays; Microbial Genetics and Genomics; Mutation; Open Reading Frames; Oryza - genetics; Physiological aspects; Plant genetics; Plant Genetics and Genomics; Proteomics; Pseudogenes; Research Article; Rice; Sequence Alignment; Sequence Analysis, DNA; Standard deviation; United States; GOSlim Term; Massively Parallel Signature Sequencing Data; Massively Parallel Signature Sequencing; Duplicate Region; Paralogous Family
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/1471-2164-10-317

Background The Osa1 Genome Annotation of rice ( Oryza sativa L. ssp. japonica cv. Nipponbare) is the product of a semi-automated pipeline that does not explicitly predict pseudogenes. As such, it is likely to mis-annotate pseudogenes as functional genes. A total of 22,033 gene models within the Osa1 Release 5 were investigated as potential pseudogenes as these genes exhibit at least one feature potentially indicative of pseudogenes: lack of transcript support, short coding region, long untranslated region, or, for genes residing within a segmentally duplicated region, lack of a paralog or significantly shorter corresponding paralog. Results A total of 1,439 pseudogenes, identified among genes with pseudogene features, were characterized by similarity to fully-supported gene models and the presence of frameshifts or premature translational stop codons. Significant difference in the length of duplicated genes within segmentally-duplicated regions was the optimal indicator of pseudogenization. Among the 816 pseudogenes for which a probable origin could be determined, 75% originated from gene duplication events while 25% were the result of retrotransposition events. A total of 12% of the pseudogenes were expressed. Finally, F-box proteins, BTB/POZ proteins, terpene synthases, chalcone synthases and cytochrome P450 protein families were found to harbor large numbers of pseudogenes. Conclusion These pseudogenes still have a detectable open reading frame and are thus distinct from pseudogenes detected within intergenic regions which typically lack definable open reading frames. Families containing the highest number of pseudogenes are fast-evolving families involved in ubiquitination and secondary metabolism.