cGMP modulates gene transcription and cation transport in Arabidopsis roots

• Published in: The Plant journal : for cell and molecular biology Vol. 45; no. 5; pp. 700 - 711
• Main Author: Maathuis, Frans J. M.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.03.2006; Blackwell Science; Blackwell Publishing Ltd
• Subjects: antiporters; Arabidopsis; Arabidopsis - physiology; Arabidopsis thaliana; barley; Biological and medical sciences; Botany; cation transport; Cation Transport Proteins; Cations; Cations, Monovalent; Cations, Monovalent - metabolism; cGMP; cyclic GMP; Cyclic GMP - physiology; Enzymes; Flowers & plants; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Plant; genes; Hordeum vulgare; ion channels; metabolism; microarray; Molecular and cellular biology; Molecular genetics; Oligonucleotide Array Sequence Analysis; physiology; Plant Roots; Plant Roots - physiology; Plant species; Potassium; Potassium - metabolism; Roots; salts; Second Messenger Systems; second messengers; signal transduction; Sodium; Sodium - metabolism; tobacco; Transcription, Genetic; Transcription. Transcription factor. Splicing. Rna processing; transcriptomics; Monocotyledones; Aleurone; Hordeum vulgare; Root; Transcription; cGMP; Metabolism; Stress; Salinity; Arabidopsis thaliana; Signal transduction; microarray; Gene; Cruciferae; Gramineae; Dicotyledones; Angiospermae; Development; transcriptomics; Spermatophyta; Chloroplast; cation transport
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1111/j.1365-313X.2005.02616.x

Summary The occurrence of the second messenger 3′,5′‐cyclic guanyl monophosphate (cGMP) has been shown in a number of plant species, including barley, tobacco and Arabidopsis. Physiological processes where cGMP signalling has been observed, or has been inferred, to play a role include chloroplast development, α‐amylase production in aleurone tissue, NO‐dependent expression of defence‐related genes and salt/osmotic stress. In most cases, it is unknown how cGMP exerts its effects and what the downstream targets are. A transcriptomics approach was therefore used to identify putative targets for cGMP signalling. Root exposure to 10 μm membrane permeable cGMP induced changes in abundance for many transcripts involved in metabolism, gene transcription, signalling and defence. In particular, monovalent cation transporters such as non‐selective ion channels and cation:proton antiporters were found to be affected in cGMP exposed roots. In addition, exposure to cGMP was found to modulate influx and efflux of the monovalent cations Na+ and K+.