KLF7联合脂肪源性干细胞对脊髓损伤大鼠运动功能的影响及其机制

目的观察KLF样转录因子7（KLF7）联合脂肪源性干细胞（ADSC）对脊髓损伤大鼠运动功能的影响,并探讨其可能的机制。方法将30只SD大鼠随机分为KLF7联合ADSC组、ADSC组及对照组,每组10只,均采用离断右半脊髓的方法制备脊髓损伤模型。KLF7联合ADSC组于脊髓损伤组织注入100μL ADSC（ADSC均采用PKH26进行标记）及2μL AAV2-KLF7腺病毒,ADSC组仅注入100μL ADSC,对照组仅注入100μL PBS。术后1天及1、2、3、4周行运动功能评分（BBB评分）;术后4周,应用诱发电位仪和磁刺激器经颅磁刺激运动诱发神经电生理反应,记录神经传导速度、潜伏期及波幅...

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Bibliographic Details
Published in山东医药 Vol. 57; no. 44; pp. 5 - 8
Main Author 李文媛;王莹;郭素芬;刘星;李智刚;刘贵波;许志鹏;张际菲
Format Magazine Article
LanguageChinese
Published 牡丹江医学院,黑龙江牡丹江,157011%牡丹江医学院红旗医院 2017
Subjects
KLF样转录因子7
大鼠
神经生长因子
脂肪源性干细胞
脊髓损伤
酪氨酸激酶A
spinal cord injury
adipose-derived stem cells
大鼠
KLF样转录因子7
nerve growth factor
脂肪源性干细胞
酪氨酸激酶A
KLF-like transcription factor 7
脊髓损伤
tyrosine kinase A
神经生长因子
rats
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ISSN1002-266X
DOI10.3969/j.issn.1002-266X.2017.44.002

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Summary:目的观察KLF样转录因子7（KLF7）联合脂肪源性干细胞（ADSC）对脊髓损伤大鼠运动功能的影响,并探讨其可能的机制。方法将30只SD大鼠随机分为KLF7联合ADSC组、ADSC组及对照组,每组10只,均采用离断右半脊髓的方法制备脊髓损伤模型。KLF7联合ADSC组于脊髓损伤组织注入100μL ADSC（ADSC均采用PKH26进行标记）及2μL AAV2-KLF7腺病毒,ADSC组仅注入100μL ADSC,对照组仅注入100μL PBS。术后1天及1、2、3、4周行运动功能评分（BBB评分）;术后4周,应用诱发电位仪和磁刺激器经颅磁刺激运动诱发神经电生理反应,记录神经传导速度、潜伏期及波幅。术后4周处死,采用Western boltting法检测脊髓损伤组织KLF7、神经生长因子（NGF）及其受体酪氨酸激酶A（TrkA）蛋白相对表达量,计算脊髓损伤面积百分比及ADSC数量（以PKH26的光密度值表示）。结果与术后1天比较,各组术后1、2、3、4周BBB评分均升高（P均〈0.05）。术后4周,KLF7联合ADSC组与ADSC组BBB评分、神经传导速度、波幅、ADSC数量及脊髓损伤组织NGF、TrkA蛋白相对表达量均高于对照组,潜伏期及脊髓损伤面积百分比均低于对照组,且KLF7联合ADSC组上述变化更明显（P均〈0.05）。KLF7联合ADSC组脊髓损伤组织KLF7蛋白相对表达量均高于ADSC组及对照组（P均〈0.05）。结论KLF7联合ADSC可减轻脊髓损伤大鼠的神经损伤,改善其运动功能;其机制可能与KLF7促进ADSC存活及提高脊髓损伤组织NGF、TrkA表达有关。
Bibliography:37-1156/R
spinal cord injury; KLF-like transcription factor 7; adipose-derived stem cells; nerve growth factor; tyro-sine kinase A; rats
LI Wenyuan1, WANG Ying, GUO Sufen, LIU Xing, LI Zhigang, LIU Guibo, XU Zhipeng, ZHANG Jifei （1 Mudanjiang Medical College, Mudanfiang 157011, China）
Objective To observe the effect of KLF7 and adipose-derived stem cell （ADSC） on motor function after spinal cord injury （SCI） in rats, and to probe the possible mechanism. Methods Thirty healthy SD rats were randomly divided into the KLF7 ＋ ADSC group, ADSC group, and control group, with 10 in each. SCI models were prepared by the method of transection of right half spinal cord. The 100 μL ADSC （ADSC labeled with PKH26） and 2 μL AAV2-KLF7 were injected into the SCI tissues in the KLF7 ＋ ADSC group, 100 μL ADSC was injected in the ADSC group, and 100 μL PBS was injected in the control group. At 1 day and 1, 2, 3, 4 weeks after operation, BBB was used to evaluate rats＇ double lower limb functional recovery in each group. At week 4 af
ISSN:1002-266X
DOI:10.3969/j.issn.1002-266X.2017.44.002