高迁移率族蛋白N2对人舌癌裸鼠移植瘤的抑瘤作用研究

目的研究高迁移率族蛋白N2（HMGN2）对人舌癌裸鼠移植瘤生长的抑瘤作用。方法裸鼠腋下注射接种舌鳞癌细胞Tca8113以建立裸鼠移植瘤模型，接种7d成瘤后，设立阴性对照组、HMGN2蛋白组和阳性对照组，在瘤体周边分别注射含washingbuffer1I、HMGN2蛋白和顺铂的细胞培养基。观察各组裸鼠肿瘤的生长情况，经过4次给药后处死裸鼠，取出瘤块，称其质量计算抑瘤率，并行苏木精一伊红（HE）染色观察形态学变化。结果成功建立舌癌裸鼠移植瘤模型。HMGN2蛋白组和阳性对照组肿瘤体积明显小于阴性对照组。裸鼠体重在整个实验过程中并没有明显的变化。阴性对照组、HMGN2蛋白组、阳性对照组的肿瘤质量平均值...

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Published in华西口腔医学杂志 Vol. 32; no. 1; pp. 5 - 8
Main Author 刘西茜 董小倩 张永红 张平 李小玉 陈思秀 冯云
Format Journal Article
LanguageChinese
Published 口腔疾病研究国家重点实验室 华西口腔医院 四川大学,成都,610041 2014
Subjects
Tca8113细胞
移植瘤
高迁移率族蛋白N2
高迁移率族蛋白N2
Tca8113细胞
transplantation tumor
high mobility group chromosomal protein N2
移植瘤
Tca8113 cell
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ISSN1000-1182
DOI10.7518/hxkq.2014.01.002

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Summary:目的研究高迁移率族蛋白N2（HMGN2）对人舌癌裸鼠移植瘤生长的抑瘤作用。方法裸鼠腋下注射接种舌鳞癌细胞Tca8113以建立裸鼠移植瘤模型，接种7d成瘤后，设立阴性对照组、HMGN2蛋白组和阳性对照组，在瘤体周边分别注射含washingbuffer1I、HMGN2蛋白和顺铂的细胞培养基。观察各组裸鼠肿瘤的生长情况，经过4次给药后处死裸鼠，取出瘤块，称其质量计算抑瘤率，并行苏木精一伊红（HE）染色观察形态学变化。结果成功建立舌癌裸鼠移植瘤模型。HMGN2蛋白组和阳性对照组肿瘤体积明显小于阴性对照组。裸鼠体重在整个实验过程中并没有明显的变化。阴性对照组、HMGN2蛋白组、阳性对照组的肿瘤质量平均值分别为（0．38±0．19）、（0．21±0．15）、（0．23±0．16）g，3组间的差异无统计学意义。HMGN2蛋白组和阳性对照组的抑瘤率分别为45．71％和39．44％。肿瘤经肉眼观察可见阴性对照组瘤块较大，HE染色可见HMGN2蛋白组和阳性对照组细胞坏死明显。结论HMGN2蛋白可以明显抑制人舌癌裸鼠移植瘤的生长。
Bibliography:high mobility group chromosomal protein N2; transplantation tumor; Tca8113 cell
51-1169/R
Liu Xiqian, Dong Xiaoqian, Zhang Yonghong, Zhang Ping, Li Xiaoyu, Chen Sixiu, Feng Yun. （State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China）
Objective To evaluate the inhibitory effect of high mobility group chromosomal protein N2 （HMGN2） on human tongue carcinoma tumor in nude mice. Methods A transplantation tumor model in nude mice was constructed by injecting Tca8113 cells. After a week, negative control groups, masculine control groups, and HMGN2 groups were esta- blished. Cell culture of the three groups were separately injected with washing buffer ]I, cis-dichlorodiamineplatinum （DDP）, and HMGN2 protein. The tumors were moved after four treatments, and then analyzed by hematoxylin-eosin （HE） staining. Results A transplanted tumor model was established successfully. The volumes of HMGN2 groups and masculine control groups were smaller than those of the n
ISSN:1000-1182
DOI:10.7518/hxkq.2014.01.002