芒柄花黄素对人胃癌细胞株MKN-45增殖、凋亡的影响及其机制

目的观察芒柄花黄素对人胃癌MKN-45细胞株增殖、凋亡的影响,并探讨其可能作用机制。方法取对数生长期人胃癌MKN-45细胞分为A、B、C、对照组,A、B、C组分别加入20、40、80μg/m L芒柄花黄素培养,对照组加入不含芒柄花黄素的完全培养基。采用MTT法观察给药24、48、72 h各组细胞增殖情况,计算细胞增殖抑制率。采用DAPI染色法评价其对MKN-45细胞形态学的影响。采用Western blotting法检测给药48 h时各组细胞磷酸化IκB（p-IκB）及NF-κB p65。结果随芒柄花黄素浓度升高,细胞增殖抑制率升高,且随干预时间增加,细胞增殖抑制率升高（P均〈0.05）。给药...

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Published in山东医药 Vol. 57; no. 7; pp. 5 - 8
Main Author 董陈诚 钟漓 张广钰 王振冉 冉福林 陈霄
Format Magazine Article
LanguageChinese
Published 广西壮族自治区人民医院,南宁,530021%桂林医学院附属医院 2017
Subjects
细胞凋亡
细胞增殖
胃癌
胃肿瘤
芒柄花黄素
细胞凋亡
cell proliferation
formononetin
芒柄花黄素
胃癌
apoptosis
胃肿瘤
stomach neoplasms
gastric carcinoma
细胞增殖
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ISSN1002-266X
DOI10.3969/j.issn.1002-266X.2017.07.002

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Summary:目的观察芒柄花黄素对人胃癌MKN-45细胞株增殖、凋亡的影响,并探讨其可能作用机制。方法取对数生长期人胃癌MKN-45细胞分为A、B、C、对照组,A、B、C组分别加入20、40、80μg/m L芒柄花黄素培养,对照组加入不含芒柄花黄素的完全培养基。采用MTT法观察给药24、48、72 h各组细胞增殖情况,计算细胞增殖抑制率。采用DAPI染色法评价其对MKN-45细胞形态学的影响。采用Western blotting法检测给药48 h时各组细胞磷酸化IκB（p-IκB）及NF-κB p65。结果随芒柄花黄素浓度升高,细胞增殖抑制率升高,且随干预时间增加,细胞增殖抑制率升高（P均〈0.05）。给药72 h时A、B、C、对照组的凋亡率分别为25.62%±2.57%、48.27%±3.18%、72.51%±4.35%、1.07%±0.54%,A、B、C组与对照组相比,P均〈0.05。给药48 h时A、B、C组细胞p-IκB、NF-κB p65蛋白相对表达量均高于对照组,且随芒柄花黄素浓度升高,细胞p-IκB及NF-κB p65蛋白相对表达量增加,P均〈0.05。结论芒柄花黄素可抑制人胃癌MKN-45细胞株的增殖、促进细胞凋亡,其机制可能为芒柄花黄素激活NF-κB信号通路。
Bibliography:37-1156/R
formononetin;gastric carcinoma;stomach neoplasms;cell proliferation;apoptosis
Objective To observe the effects of formononetin on the proliferation and apoptosis of human gastric cancer MKN-45 cells and its possible mechanism. Methods The human gastric cancer MKN-45 cells in the logarithmic growth phase were divided into groups A,B,C and the control group. Cells in the groups A,B and C were cultured and the culture medium was added with 20,40 and 80 μg / m L of formononetin,respectively. The control group was not treated. The cell proliferation of MKN-45 cells was detected by MTT assay at 24,48 and 72 h of formononetin treatment,and the growth inhibitory rate of MKN-45 cells was assessed. The effect of formononetin on morphological changes was evaluated using DAPI staining. The intracellular phosphorylated IκB（ p-IκB） and NF-κB p65 protein expression was measured by Western blotting. Results MKN-45 cell proliferation was inhibited after being treated by formononetin with a time- and dose-dependent man
ISSN:1002-266X
DOI:10.3969/j.issn.1002-266X.2017.07.002